Gene Expression And Transcription | Research Area | St John's Laboratory

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tip60 (Phospho-Ser90) Antibody

Anti-Phospho-KAT5-Ser90 antibody (56-105 aa) (STJ91271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of SH-SY5Y 293T 3T3 lysis using Phospho-DREAM (S63) antibody. Antibody was diluted at 1:500

Anti-Phospho-KCNIP3-Ser63 antibody (29-78 aa) (STJ91090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-KIT-Tyr568/570 antibody (STJ196274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using KIT (Phospho-Tyr703) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-KIT-Tyr703 antibody (671-720 aa) (STJ90818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-Phospho-KIT-Tyr719 antibody (STJ196275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-c-Kit (Y721) Polyclonal Antibody

Anti-Phospho-KIT-Tyr721 antibody (688-737 aa) (STJ90226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-KIT-Tyr823 antibody (STJ196276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-KIT-Tyr936 antibody (906-955 aa) (STJ90817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (STJ22223). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-KIT-Y721 antibody (STJ22223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of various lysates using Phospho-KRAS-S89 Rabbit pAb (STJ11102656) at 1:500 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-KRAS-S89 antibody (STJ11102656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of 293 cells using Phospho-LEF-1 (S42) Polyclonal Antibody

Anti-Phospho-LEF1-Ser42 antibody (8-57 aa) (STJ90534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from HL60 cells, using Phospho-MEK6-S207 Rabbit pAb (STJ22247). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K6-S207 antibody (STJ22247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MAP2K6-Ser207 antibody (STJ90333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-MAPK1 antibody [M206] (STJA0005411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€488.13

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Anti-Phospho-MAPK14 antibody (250-300) (STJ502555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Anti-Phospho-MAPK14 antibody (250-300) {Biotin} (STJ502556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€730.63

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Anti-Phospho-MAPK14 antibody (250-300) {FITC} (STJ502557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€730.63

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-p38 MAPK-Y182 Rabbit polyclonal antibody (STJ11105887) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180 antibody (STJ11105887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemistry analysis of Phospho-p38 MAPK-T180/Y182 in paraffin-embedded Mouse kidney using Phospho-p38 MAPK-T180/Y182 Rabbit polyclonal antibody (STJ11105812) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180/Y182 antibody (STJ11105812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180 antibody (147-196 aa) (STJ90362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180/Y182 antibody (151-200 aa) (STJ90498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/FC/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Guinea Pig

€60.00 - €315.00

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Western blot analysis of lysates from 1) PC-3, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-MAPK14-Tyr182 antibody (147-196 aa) (STJ90363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of human-stomach tissue. 1, p38 (phospho Tyr323) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-MAPK14-Tyr323 antibody (288-337 aa) (STJ90878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (STJ22257) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-MAPK14-Y182 antibody (STJ22257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 + ERK2-Y187 Rabbit pAb (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MAPKAP1-Thr86 antibody (STJ196423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-MAPKAPK5-Thr182 antibody (148-197 aa) (STJ90980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-MAX-S11 Rabbit pAb (STJ22282) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAX-S11 antibody (STJ22282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Anti-Phospho-MAX-Ser11 antibody (STJA0009824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-MAX-Ser2 antibody (1-50 aa) (STJ91169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MDC1-Ser513 antibody (479-528 aa) (STJ91260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S166 Rabbit pAb (STJ22283). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MDM2-S166 antibody (STJ22283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-MDM2-S166 Rabbit monoclonal antibody (STJ11105811) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MDM2-S166 antibody [S5811RM] (STJ11105811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S260 Rabbit pAb (STJ22284). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MDM2-S260 antibody (STJ22284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Immunofluorescence analysis of Hela cell. 1, MDM2 (phospho Ser166) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HAO1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-MDM2-Ser166 antibody (132-181 aa) (STJ91043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MDM2-Ser186/S188 antibody (151-200 aa) (STJ91352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of HELA-UV Cell Lysate using antibody

Anti-Phospho-MDM2-Tyr394 antibody (STJ98946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MDM4 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MDM4-Ser367 antibody (336-385 aa) (STJ90981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-MED1-Thr1032 antibody (STJA0010021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MED1-Thr1457 antibody (1423-1472 aa) (STJ91050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€60.00 - €315.00

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Western blot analysis of lysates from K-562 and MCF-7 cells, using Phospho-MEF2C-S396 Rabbit pAb (STJ22289) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MEF2C-S396 antibody (STJ22289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-MEF2C-Ser222 antibody (STJA0003682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Chicken/Guinea Pig/Non-Human Primates/Mouse/Rat/Sheep

€568.13

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Immunohistochemistry analysis of paraffin-embedded human heart, using MEF2C (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2C-Ser387 antibody (353-402 aa) (STJ91116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using MEF2C (Phospho-Ser396) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2C-Ser396 antibody (362-411 aa) (STJ90982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MET-Ser985 antibody (STJA0010082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-Met (Phospho-Tyr1003) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MET-Tyr1003 antibody (976-1025 aa) (STJ91076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-Met (Y1234) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MET-Tyr1234 antibody (1201-1250 aa) (STJ90334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MET-Tyr1234/1235 antibody (STJ196357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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