• Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MDM4 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MDM4 (Phospho-Ser367) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MDM4-Ser367 antibody (336-385 aa) (STJ90981)

SKU:
STJ90981

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein Mdm4-Ser367 (336-385 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDM4
Gene ID: 4194
Uniprot ID: MDM4_HUMAN
Immunogen Region: 336-385 aa
Specificity: Phospho-MDMX (S367) Polyclonal Antibody detects endogenous levels of MDMX protein only when phosphorylated at S367.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MDM4 around the phosphorylation site of Ser367 at the amino acid range 336-385
Post Translational Modifications Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent. Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein.
Function Along with MDM2, contributes to TP53 regulation. Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Protein Name Protein Mdm4
Double Minute 4 Protein
Mdm2-Like P53-Binding Protein
Protein Mdmx
P53-Binding Protein Mdm4
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-5689880
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-6804760
Reactome: R-HSA-69541
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Mdm4 antibody
Anti-Double Minute 4 Protein antibody
Anti-Mdm2-Like P53-Binding Protein antibody
Anti-Protein Mdmx antibody
Anti-P53-Binding Protein Mdm4 antibody
Anti-MDM4 antibody
Anti-MDMX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance