Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Cyclin D2 antibody (STJ26178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCND2 antibody (1-289) (STJ26178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from 293 cells, using Cyclin D2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCND2 antibody (240-289 aa) (STJ92539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot detection of Cyclin D2 in CHO-K1 cell lysate (A) and CHO-K1 transfected by Cyclin D2-fragment EGFP fusion protein (B) cell lysate using Cyclin D2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-CCND2 antibody [6E11-G6-F5] (STJ99098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$205.62 - $496.80

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Western blot analysis of lysates from wild type (WT) and Cyclin D3 knockout (KO) HeLa cells, using [KO Validated] Cyclin D3 Rabbit polyclonal antibody (STJ22949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CCND3 antibody (201-292) (STJ22949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of extracts of Rat lung, using Cyclin D3 Rabbit monoclonal antibody (STJ11101980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CCND3 antibody (202-292) [S0MR] (STJ11101980)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of COS7 cells using Cyclin D3 Polyclonal Antibody

Anti-CCND3 antibody (243-292 aa) (STJ92540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis using Cyclin D3 monoclonal antibody against NIH/3T3 (1) and Jurkat (2) cell lysate.

Anti-CCND3 antibody [6H4] (STJ97979)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$205.62 - $496.80

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Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (STJ116441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCNE1 antibody (300-400) (STJ116441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of Cyclin E1 in paraffin-embedded mouse brain using Cyclin E1 Rabbit monoclonal antibody (STJ11104815) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CCNE1 antibody (300-400) [S4815RM] (STJ11104815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of Hela cell. 1, Cyclin E1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CCNE1 antibody (91-140 aa) (STJ92541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

$67.62 - $360.18

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Anti-CCNE1 antibody (N-Term) (STJA0005672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$674.13

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Immunohistochemistry analysis of Cyclin E2 in paraffin-embedded mouse testis using Cyclin E2 Rabbit polyclonal antibody (STJ22953) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCNE2 antibody (255-404) (STJ22953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of MCF7 cells using Cyclin E2 antibody (STJ29112).

Anti-CCNE2 antibody (255-404) (STJ29112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of NIH/3T3 cells, using Cyclin E2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CCNE2 antibody (355-404 aa) (STJ92544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using Cyclin E2 Rabbit mAb (STJ11102474) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-CCNE2 antibody (50-150) [S4MR] (STJ11102474)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-CCNE2 antibody (N-Term) (STJA0005671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$674.13

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Western blot analysis of various cells using Cyclin G Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CCNG1 antibody (161-210 aa) (STJ92546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded mouse testis using Cyclin G1 antibody (STJ27245) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCNG1 antibody (200-295) (STJ27245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCNG2 antibody (20-100 aa) (STJ191665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using Cyclin H antibody (STJ11104592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCNH antibody (1-100) [S4592RM] (STJ11104592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of various lysates using Cyclin H Rabbit polyclonal antibody (STJ11104489). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-CCNH antibody (1-323) (STJ11104489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunoprecipitation analysis of 200 Mu g extracts of K562 cells using 1 Mu g Cyclin H antibody (STJ22956). Western blot was performed from the immunoprecipitate using Cyclin H antibody (STJ22956) at a dilution of 1:1000.

Anti-CCNH antibody (1-323) (STJ22956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit monoclonal antibody (STJ11101991) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CCNH antibody (224-323) [S1MR] (STJ11101991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of various cells using Cyclin H Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CCNH antibody (274-323 aa) (STJ92547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis using Cyclin H monoclonal antibody against Jurkat, K562, A431 cell lysate.

Anti-CCNH antibody (STJ98481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Dog/Pig

$205.62 - $496.80

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Western blot detection of Cyclin H in Jurkat, K562 and A431 cell lysates using Cyclin H mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa.

Anti-CCNH antibody [4E11-G2-D7] (STJ99112)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

$205.62 - $496.80

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Immunohistochemistry analysis of paraffin-embedded rat brain using Cyclin K Rabbit polyclonal antibody (STJ112299) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CCNK antibody (1-160) (STJ112299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western Blot analysis of HEK293 lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-CCNK antibody (80-160 aa) (STJ191667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-CCNT1 antibody (220-300 aa) (STJ191669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates, using Cyclin T1 Rabbit polyclonal antibody (STJ22961) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCNT1 antibody (220-320) (STJ22961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-CCNT2 antibody (510-590 aa) (STJ191670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CCNT2 antibody (510-590 aa) (STJ193202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-CD103 antibody [B-ly7] (STJA0018348)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$1,978.23

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Anti-CD103 antibody [B-ly7] {FITC} (STJA0018347)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$3,205.05

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Anti-CD103 antibody [B-ly7] {R-PE} (STJA0018349)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$3,841.23

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Anti-CD115 antibody [R09-5G-2] (STJA0033146)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$296.01 - $425.73

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Anti-CD115/CSF1R antibody [FPA-008] (STJA0026940)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$351.21 - $435.39

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Anti-CD115/CSF1R antibody [FPA-008] {APC} (STJA0029044)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$428.49 - $540.27

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Anti-CD115/CSF1R antibody [FPA-008] {FITC} (STJA0028078)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$351.21 - $435.39

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Anti-CD115/CSF1R antibody [FPA-008] {PerCP} (STJA0030010)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$403.65 - $504.39

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Anti-CD115/CSF1R antibody [FPA-008] {PE} (STJA0030976)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

$428.49 - $540.27

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Anti-CD115/CSF1R antibody [RG7155] (STJA0026941)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$351.21 - $435.39

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Anti-CD115/CSF1R antibody [RG7155] {APC} (STJA0029045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$428.49 - $540.27

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Anti-CD115/CSF1R antibody [RG7155] {FITC} (STJA0028079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$351.21 - $435.39

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Anti-CD115/CSF1R antibody [RG7155] {PerCP} (STJA0030011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$403.65 - $504.39

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Anti-CD115/CSF1R antibody [RG7155] {PE} (STJA0030977)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$428.49 - $540.27

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Formalin-fixed, paraffin-embedded human GIST stained with anti-CD117 antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells

Anti-CD117 antibody (50-250aa) [ZM321] (STJ180553)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$202.17 - $748.65

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Anti-CD117 antibody [104D2] {EF700} (STJA0042939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$218.73 - $446.43

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