Anti-CCND2 antibody (1-289) (STJ26178)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cyclin D2 (1-289) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCND2 |
| Gene ID | 894 |
| Uniprot ID | CCND2_HUMAN |
| Immunogen Region | 1-289 |
| Immunogen Sequence | MELLCHEVDPVRRAVRDRNL LRDDRVLQNLLTIEERYLPQ CSYFKCVQKDIQPYMRRMVA TWMLEVCEEQKCEEEVFPLA MNYLDRFLAGVPTPKSHLQL LGAVCMFLASKLKETSPLTA EKLCIYTDNSIKPQELLEWE LVVLGKLKWNLAAVTPHDFI EHILRKLPQQREKLSLIRKH AQTFIALCATDFKFAMYPPS MIATGSVGAAICGLQQDEEV SSLTCDALTELLAKITNTD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human Cyclin D2 (NP_001750.1). |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-280 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex. Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-280 phosphorylation. The DCX(AMBRA1) complex represents the major regulator of CCND2 stability during the G1/S transition. Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation. |
| Function | Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. |
| Protein Name | G1/S-Specific Cyclin-D2 |
| Database Links | Reactome: R-HSA-69231Reactome: R-HSA-8934593Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | NucleusCytoplasmNucleus MembraneCyclin D-Cdk4 Complexes Accumulate At The Nuclear Membrane And Are Then Translocated Into The Nucleus Through Interaction With Kip/Cip Family MembersIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D2 antibodyAnti-CCND2 antibody |
Information sourced from Uniprot.org