Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various cells using DR4 Polyclonal Antibody

Anti-TNFRSF10A antibody (401-450 aa) (STJ92775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Anti-TNFRSF10B antibody (200-280) (STJA0010101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of Jurkat cells, using DR5/TRAILR2/TNFRSF10B antibody (STJ25885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-TNFRSF10B antibody (260-440) (STJ25885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Confocal imaging of NIH-3T3 cells using DR5/TRAILR2/TNFRSF10B Rabbit monoclonal antibody (STJ11101581, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-TNFRSF10B antibody (341-440) [S1MR] (STJ11101581)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot detection of DR5 in Mouse kindey, Mouse brain, HCT116 and Hela cell lysates using DR5 mouse mAb (1:500-1:2000 diluted).Predicted band size:40/48KDa.Observed band size:40/48KDa.

Anti-TNFRSF10B antibody [7F4-F8-G11] (STJ99180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of NIH-3T3, PC12 cells using DcR1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-TNFRSF10C antibody (11-60 aa) (STJ96848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using DcR1/ CD263 Rabbit monoclonal antibody (STJ11105148) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TNFRSF10C antibody (150-230) [S5148RM] (STJ11105148)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using TNFRSF10C antibody (STJ25886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180.

Anti-TNFRSF10C antibody (150-238) (STJ25886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using DcR1/CD263 Rabbit monoclonal antibody (STJ11105220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : K-562Exposure time: 45s.

Anti-TNFRSF10C antibody (26-235) [S5220RM] (STJ11105220)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-TNFRSF10C antibody (N-Term) (STJA0005753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Western blot analysis of TNFRSF10D Antibody. The lane on the right is blocked with the TNFRSF10D peptide.

Anti-TNFRSF10D antibody (121-170 aa) (STJ92107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using TNFRSF10D Rabbit pAb (STJ116013) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 20s.

Anti-TNFRSF10D antibody (257-386) (STJ116013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using TNFRSF10D Rabbit polyclonal antibody (STJ27889) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-TNFRSF10D antibody (257-386) (STJ27889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using GITR/TNFRSF18 Rabbit polyclonal antibody (STJ118073) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TNFRSF18 antibody (26-255) (STJ118073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TNFRSF18 antibody (STJ192966)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using TAB182 Polyclonal Antibody diluted at 1：1000

Anti-TNKS1BP1 antibody (1601-1650 aa) (STJ95885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat stomach using TNKS1BP1 Rabbit polyclonal antibody (STJ11103972) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-TNKS1BP1 antibody (700-1000) (STJ11103972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using TNRC6A antibody (STJ29936) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TNRC6A antibody (950-1050) (STJ29936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-TNRC6A antibody (STJA0009795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-TNRC6C antibody (784-834 aa) (STJ197640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293T using Topo III Alpha antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-TOP3A antibody (350-430 aa) (STJ97619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using TOPBP1 Rabbit pAb (STJ114440) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 50s.

Anti-TOPBP1 antibody (1253-1522) (STJ114440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-TOPBP1 antibody (STJA0010274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of p53 (green) in MCF-7 using p53 antibody, and DAPI (blue)

Anti-TP53 antibody (1-101 aa) [R07-6A7] (STJA0011734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g p53 antibody (STJ11104447). Western blot was performed from the immunoprecipitate using p53 antibody (STJ11104447) at a dilution of 1:3000.

Anti-TP53 antibody (1-393) (STJ11104447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g p53 antibody (STJ11104449). Western blot was performed from the immunoprecipitate using p53 antibody (STJ11104449) at a dilution of 1:2000.

Anti-TP53 antibody (1-393) (STJ11104449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation of p53 in 200 µg extracts from 293T cells using 3 µg p53 Rabbit polyclonal antibody (STJ25926). Western blot analysis was performed using p53 Rabbit polyclonal antibody (STJ25926) at 1:3000 dilution.

Anti-TP53 antibody (1-393) (STJ25926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of U-251MG cells using 3 Mu g p53 Rabbit polyclonal antibody (STJ25927). Western blot was performed from the immunoprecipitate using p53 Rabbit polyclonal antibody (STJ25927) at a dilition of 1:1000.

Anti-TP53 antibody (1-393) (STJ25927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] p53 Rabbit monoclonal antibody (STJ11101713). Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit monoclonal antibody (STJ11101713) at a dilution of 1:1000.

Anti-TP53 antibody (1-393) [S3MR] (STJ11101713)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of lysates from 1) p53 knockout A431 cell, 2) A431 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-TP53 antibody (10-59 aa) (STJ94893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of U-251MG cells using 3 Mu g p53 antibody (STJ28328). Western blot was performed from the immunoprecipitate using p53 antibody (STJ28328) at a dilution of 1:1000.

Anti-TP53 antibody (218-390) (STJ28328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Human esophageal squamous cell carcinoma tissue was stained with Anti-P53 (ABT-P53) Antibody

Anti-TP53 antibody (250-393 aa) [ABT-P53] (STJ197083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53 (ABT002) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 53kDaObserved band size: 53kDa

Anti-TP53 antibody (250-393 aa) [ABT002] (STJ197180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-P53 antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TP53 antibody (280aa C-Term) (STJ140047)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Anti-P53 antibody at 1:2500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TP53 antibody (280aa C-Term) (STJ140114)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TP53 antibody (283-332 aa) (STJ94905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various cells using p53 Polyclonal Antibody diluted at 1：1000

Anti-TP53 antibody (301-350 aa) (STJ94894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha (phospho Ser51) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TP53 antibody [6C4] (STJ96954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-TP53 antibody [ABT239R] (STJ197050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Human breast carcinoma infiltrating fat stained with anti-p53 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of carcinoma cells.

Anti-TP53 antibody [DO‐7] (STJ180207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £481.50

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Anti-TP53 antibody [Pab122] (STJ16100763)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Dog/Human/Monkey/Mouse/Rat

£287.50 - £1,324.50

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Western blot analysis of various lysates using p53 Mouse monoclonal antibody (STJ112620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TP53 antibody [S0MM] (STJ112620)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from 293T cells using [KO Validated] p53 Rabbit monoclonal antibody (STJ11105127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TP53 antibody [S5127RM] (STJ11105127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-TP53/p53 (R175H mutant) antibody [F2] (STJA0027800)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-TP53/p53 (R175H mutant) antibody [F2] {APC} (STJA0028999)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Anti-TP53/p53 (R175H mutant) antibody [F2] {FITC} (STJA0028033)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-TP53/p53 (R175H mutant) antibody [F2] {PerCP} (STJA0029965)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£265.50 - £396.50

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Anti-TP53/p53 (R175H mutant) antibody [F2] {PE} (STJA0030931)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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