Gene Expression And Transcription | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-POLR3B antibody (321-370 aa) (STJ95179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from rat ovary, using POLR3C Rabbit polyclonal antibody (STJ118229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-POLR3C antibody (1-270) (STJ118229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of K562 cells using POLR3C Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLR3C antibody (191-240 aa) (STJ95180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from MCF-7 cells, using RPC4 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-POLR3D antibody (281-330 aa) (STJ95181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLR3E antibody (191-240 aa) (STJ95182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using POLR3F Rabbit polyclonal antibody (STJ114115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-POLR3F antibody (1-220) (STJ114115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR3F antibody (STJ190767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of PC12 cells using POLR3G Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLR3G antibody (151-200 aa) (STJ96854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of K562 cells using POLR3H Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLR3H antibody (151-200 aa) (STJ95183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using POLR3K antibody (STJ28550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLR3K antibody (1-108) (STJ28550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR3K antibody (51-100 aa) (STJ190766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g POLRMT antibody (STJ118054). Western blot was performed from the immunoprecipitate using POLRMT antibody (STJ118054) at a dilution of 1:1000.

Anti-POLRMT antibody (42-230) (STJ118054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

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Anti-POLRMT antibody (STJ190769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Polycomb Protein SUZ12 antibody [R02-9F5] (STJA0036221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Polycomb Protein SUZ12 antibody [R07-6C3] (STJA0035373)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-Polyubiqutin-B antibody [Ubi-1] (STJA0040757)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/WB

Reactivity :

Bovine/C.elegans/Chicken/Drosophila/Human

$729.33

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Western blot analysis of 293F-POMC-His-C, using POMC rabbit polyclonal antibody (STJ25054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POMC antibody (1-100) (STJ25054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Human pituitary tissue was stained with anti-ACTH (ABT-ACTH) antibody.

Anti-POMC antibody (100-200 aa) [ABT-ACTH] (STJ196740)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-POMC antibody (STJ193173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-POMC antibody [6D4-7B1-1A1] (STJA0033420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$296.01 - $425.73

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Anti-POMC antibody [R02-2J8] (STJA0036591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of lysates from rat kidney, using POU2F1/OCT1 Rabbit polyclonal antibody (STJ25059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-POU2F1 antibody (15-290) (STJ25059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$152.49 - $697.59

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Western blot analysis of various cells using Oct-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POU2F1 antibody (41-90) (STJ94595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-POU2F1/OCT1 antibody (1-162aa) [SRM13604] (STJ11108334)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various cells using Oct-2 Polyclonal Antibody

Anti-POU2F2 antibody (1-50) (STJ94596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$67.62 - $360.18

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Human diffuse large B-cell lymphoma tissue was stained with Anti-Oct-2 (ABT211) Antibody

Anti-POU2F2 antibody (250-350 aa) [ABT211] (STJ197093)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse

$248.40 - $360.18

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Western blot analysis of extracts of various cell lines, using POU2F2 antibody (STJ11104267) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POU2F2 antibody (400-479) (STJ11104267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of rat eye cells using POU4F1Rabbit polyclonal antibody (STJ11104894) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-POU4F1 antibody (1-100 aa) (STJ11104894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-POU4F1 antibody (1-100 aa) [SRM5294] (STJ11107196)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POU4F1 antibody (311-360 aa) (STJ91894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of U-2 OS cells using POU4F2 Rabbit polyclonal antibody (STJ11103925) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-POU4F2 antibody (1-100) (STJ11103925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-POU4F2 antibody (STJ198354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-PP2A-gamma antibody (390-439) (STJ505956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-PP2A-gamma antibody (390-439) {Biotin} (STJ505957)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-PP2A-gamma antibody (390-439) {FITC} (STJ505958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-PP2C alpha/PPM1A antibody (1-382aa) [p6c7] (STJA0041531)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$302.91 - $458.85

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Anti-PP2C alpha/PPM1A antibody (1-382aa) [p6C7] (STJA0041902)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$329.13 - $498.87

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Anti-PPAR delta antibody [4F4-3B6-7D1] (STJA0032982)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Anti-PPAR delta antibody [4G5-2D10-2H5] (STJA0032980)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Anti-PPAR Gamma antibody (STJ11107753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-PPAR gamma antibody (STJA0037486)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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Anti-PPAR gamma antibody [R11-4O-6] (STJA0035454)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$184.23 - $388.47

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Anti-PPAR gamma/NR1C3 antibody (209-477aa) [AT4F5] (STJA0041887)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

$302.91 - $458.85

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Western blot analysis of various lysates using PPAR Alpha Rabbit pAb (STJ28780) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-PPARA antibody (1-92) (STJ28780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Western blot analysis of lysates from Mouse kidney using PPARA Rabbit polyclonal antibody (STJ11105478) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPARA antibody (201-300) (STJ11105478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse

$152.49 - $697.59

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Immunofluorescence analysis of Hela cell. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-PPARA antibody (6-55 aa) (STJ95200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Rat brain using PPARD Rabbit pAb (STJ11107104) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit (

Anti-PPARD antibody (101-200 aa) (STJ11107104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded rat liver using PPARD Rabbit polyclonal antibody (STJ27623) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PPARD antibody (102-361) (STJ27623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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