This YWHAz Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma, tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Rat
Sensitivity
0.057ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Highly expressed in brain (at protein level).
Post Translational Modifications
The delta, brain-specific form differs from the zeta form in being phosphorylated. Phosphorylation on Ser-184 by MAPK8.promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232.inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA.disrupts homodimerization and heterodimerization with YHAE and TP53.
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
Protein Name
14-3-3 Protein Zeta/DeltaMitochondrial Import Stimulation Factor S1 SubunitProtein Kinase C Inhibitor Protein 1Kcip-1
