| Host: |
Rabbit/Rabbit |
| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This VEGFR-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Clonality: |
Monoclonal/Polyclonal |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Purification: |
Capture Purification: Protein A.Detection Purification: Protein A & Antigen Affinity. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: |
Kdr |
| Gene ID: |
25589 |
| Uniprot ID: |
VGFR2_RAT |
| Immunogen: |
Capture immunogen: Recombinant Rat VEGFR-2 protein.Detection Immunogen: Recombinant Rat VEGFR-2 protein. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-947 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1171 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1210 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-797, Tyr-947, Tyr-992, Tyr-1050, Tyr-1055, Tyr-1171 and Tyr-1210. The inhibitory disulfide bond between Cys-1020 and Cys-1041 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 2Vegfr-2Fetal Liver Kinase 1Flk-1Protein-Tyrosine Kinase Receptor Flk-1Cd Antigen Cd309 |
| Database Links | Reactome: R-RNO-194306Reactome: -RNO-195399Reactome: -RNO-4420097Reactome: -RNO-5218921 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmNucleusCytoplasmic VesicleEarly EndosomeCell JunctionEndoplasmic ReticulumDetected On Caveolae-Enriched Lipid Rafts At The Cell SurfaceIs Recycled From The Plasma Membrane To Endosomes And Back AgainPhosphorylation Triggered By Vegfa Binding Promotes Internalization And Subsequent DegradationVegfa Binding Triggers Internalization And Translocation To The NucleusLocalized With Rap1a At Cell-Cell JunctionsColocalizes With Ern1 And Xbp1 In The Endoplasmic Reticulum In Endothelial Cells In A Vascular Endothelial Growth Factor (Vegf)-Dependent Manner |
| Alternative Antibody Pair Names | Vascular Endothelial Growth Factor Receptor 2 antibody pairVegfr-2 antibody pairFetal Liver Kinase 1 antibody pairFlk-1 antibody pairProtein-Tyrosine Kinase Receptor Flk-1 antibody pairCd Antigen Cd309 antibody pairKdr antibody pairFlk1 antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
