Rat VEGF-A/VEGF164 protein (Recombinant) (C-His) (STJP001318)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Rat VEGF-A/VEGF164 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat VEGF-A/VEGF164-C-His protein was developed in hek293 cells using the region Ala27-Arg190. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Rat VEGF164 at 1 Mu g/mL (100 Mu L/well) can bind Human KDR with a linear range of 0.03-3.6 ng/mL.|2.Measured in a cell proliferation assay using human umbilical vein endothelial ce |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Vegfa |
| Gene ID | 83785 |
| Uniprot ID | VEGFA_RAT |
| Immunogen | Recombinant Rat VEGF-A/VEGF164 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6×His tag at the C-terminus. |
| Immunogen Region | Ala27-Arg190 |
| Immunogen Sequence | APTTEGEQKAHEVVKFMDVY QRSYCRPIETLVDIFQEYPD EIEYIFKPSCVPLMRCAGCC NDEALECVPTSESNVTMQIM RIKPHQSQHIGEMSFLQHSR CECRPKKDRTKPENHCEPCS ERRKHLFVQDPQTCKCSCKN TDSRCKARQLELNERTCRCD KPRR |
Additional Info
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. |
| Protein Name | Vascular Endothelial Growth Factor AVegf-AVascular Permeability FactorVpf |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-194313Reactome: -RNO-195399Reactome: -RNO-4420097Reactome: -RNO-5218921 |
| Cellular Localisation | SecretedVegf-A120 Is Acidic And Freely SecretedVegf-A164 Is More BasicHas Heparin-Binding Properties AndAlthough A Significant Proportion Remains Cell-AssociatedMost Is Freely SecretedVegf-A188 Is Very BasicIt Is Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative Protein Names | Vascular Endothelial Growth Factor A proteinVegf-A proteinVascular Permeability Factor proteinVpf proteinVegfa proteinVegf protein |
Information sourced from Uniprot.org