Rat UCP2 (Uncoupling Protein 2, Mitochondrial) Sandwich ELISA Kit Ready-To-Use Kit (STJE0019305)
SPECIFICATIONS
General Information
| Short Description | This UCP2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 0.055ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Ucp2 |
| Gene ID | 54315 |
| Uniprot ID | UCP2_RAT |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in a variety of organs, with predominant expression in the heart, lung and spleen. |
| Post Translational Modifications | |
| Function | Antiporter that exports dicarboxylate intermediates of the Krebs cycle in exchange for phosphate plus a proton across the inner membrane of mitochondria, a process driven by mitochondrial motive force with an overall impact on glycolysis, glutaminolysis and glutathione-dependent redox balance. Continuous export of oxaloacetate and related four-carbon dicarboxylates from mitochondrial matrix into the cytosol negatively regulates the oxidation of acetyl-CoA substrates via the Krebs cycle lowering the ATP/ADP ratio and reactive oxygen species (ROS) production. May mediate inducible proton entry into the mitochondrial matrix affecting ATP turnover as a protection mechanism against oxidative stress. The proton currents are most likely associated with fatty acid flipping across the inner membrane of mitochondria in a metabolic process regulated by free fatty acids and purine nucleotides. Regulates the use of glucose as a source of energy. Required for glucose-induced DRP1-dependent mitochondrial fission and neuron activation in the ventromedial nucleus of the hypothalamus (VMH). This mitochondrial adaptation mechanism modulates the VMH pool of glucose-excited neurons with an impact on systemic glucose homeostasis. Regulates ROS levels and metabolic reprogramming of macrophages during the resolution phase of inflammation. Attenuates ROS production in response to IL33 to preserve the integrity of the Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. Can unidirectionally transport anions including L-malate, L-aspartate, phosphate and chloride ions. Does not mediate adaptive thermogenesis. |
| Protein Name | Dicarboxylate Carrier Ucp2Mitochondrial Uncoupling Protein 2Ucp 2Solute Carrier Family 25 Member 8 |
| Database Links | Reactome: R-RNO-167826 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Dicarboxylate Carrier Ucp2 ELISA kitMitochondrial Uncoupling Protein 2 ELISA kitUcp 2 ELISA kitSolute Carrier Family 25 Member 8 ELISA kitUcp2 ELISA kitSlc25a8 ELISA kit |
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Information sourced from Uniprot.org