Rat Shbg (Sex hormone-binding globulin) Sandwich ELISA Kit (STJE0006096)

SPECIFICATIONS
STJE0006096
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General Information

Short DescriptionThis Shbg Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityRat
Sensitivity9.38pg/mL
Detection Limit15.63~1000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolShbg
Gene ID24775
Uniprot IDSHBG_RAT
SpecificityThis kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Protein Name Sex Hormone-Binding Globulin
Shbg
Sex Steroid-Binding Protein
Sbp
Testis-Specific Androgen-Binding Protein
Abp
Database Links
Cellular Localisation Secreted
In Testis
It Is Synthesized By The Sertoli Cells
Secreted Into The Lumen Of The Seminiferous Tubule And Transported To The Epididymis
Alternative ELISA Names Sex Hormone-Binding Globulin ELISA kit
Shbg ELISA kit
Sex Steroid-Binding Protein ELISA kit
Sbp ELISA kit
Testis-Specific Androgen-Binding Protein ELISA kit
Abp ELISA kit
Shbg ELISA kit
Specificity This kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 100
EDTA plasma (n=8) 91-104 97
Cell culture media (n=8) 87-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.63 141.90 414.51 47.89 130.99 431.49
Standard deviation 2.60 6.90 15.30 3.14 6.48 22.78
CV (%) 5.04 4.86 3.69 6.56 4.95 5.28

Citations

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