Rat SEPP1 (Selenoprotein P) CLIA Kit for Quantitative Detection

Name: Rat SEPP1 (Selenoprotein P) CLIA Kit for Quantitative Detection
SKU: STJC0001878
Availability: InStock

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SPECIFICATIONS

STJC0001878

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Short Description	This rat SEPP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Rat
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	Selenop
Gene ID	29360
Uniprot ID	SEPP1_RAT
Specificity	This kit recognizes Rat SEPP1 in samples. No significant cross-reactivity or interference between Rat SEPP1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Widely expressed, mainly by the liver. Secreted in plasma.
Post Translational Modifications	Isoform Se-P1 contains several disulfide bridges and a selenide-sulfide bond between Sec-59 and Cys-62. These bonds are speculated to serve as redox-active pairs. Phosphorylation sites are present in the extracellular medium.
Function	Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
Protein Name	Selenoprotein P Sep Cleaved Into - Selenoprotein Se-P10 - Selenoprotein Se-P6 - Selenoprotein Se-P2 - Selenoprotein Se-P1
Database Links	Reactome: R-RNO-114608
Cellular Localisation	Secreted Passes From Plasma Into The Glomerular Filtrate Where It Is Removed By Endocytosis Mediated By Lrp2 In The Proximal Tubule Epithelium
Alternative CLIA Names	Selenoprotein P CLIA kit Sep Cleaved Into - Selenoprotein Se-P10 - Selenoprotein Se-P6 - Selenoprotein Se-P2 - Selenoprotein Se-P1 CLIA kit Selenop CLIA kit Selp CLIA kit Sepp1 CLIA kit
Specificity	This kit recognizes Rat SEPP1 in samples. No significant cross-reactivity or interference between Rat SEPP1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	95.94	243.48	697.14	92.31	219.58	762.37
Standard deviation	12.45	18.77	76.34	8.78	15.66	84.47
CV (%)	12.98	7.71	10.95	9.51	7.13	11.08