Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) Sandwich ELISA Kit (STJE0006068)

Name: Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) Sandwich ELISA Kit (STJE0006068)
SKU: STJE0006068
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0006068

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General Information

Short Description	This RANkL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	Tnfsf11
Gene ID	117516
Uniprot ID	TNF11_RAT
Specificity	This kit recognizes Rat RAN Kappa L in samples. No significant cross-reactivity or interference between Rat RAN Kappa L and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	The soluble form derives from the membrane form by proteolytic processing.
Function	Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 11 Osteoclast Differentiation Factor Odf Osteoprotegerin Ligand Opgl Receptor Activator Of Nuclear Factor Kappa-B Ligand Rankl Tnf-Related Activation-Induced Cytokine Trance Cd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form
Database Links	Reactome: R-RNO-5668541 Reactome: -RNO-5669034 Reactome: -RNO-5676594
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Ligand Superfamily Member 11 Soluble Form: Secreted
Alternative ELISA Names	Tumor Necrosis Factor Ligand Superfamily Member 11 ELISA kit Osteoclast Differentiation Factor ELISA kit Odf ELISA kit Osteoprotegerin Ligand ELISA kit Opgl ELISA kit Receptor Activator Of Nuclear Factor Kappa-B Ligand ELISA kit Rankl ELISA kit Tnf-Related Activation-Induced Cytokine ELISA kit Trance ELISA kit Cd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form ELISA kit Tnfsf11 ELISA kit Opgl ELISA kit Rankl ELISA kit Trance ELISA kit
Specificity	This kit recognizes Rat RANκL in samples. No significant cross-reactivity or interference between Rat RANκL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-103	96
EDTA plasma (n=8)	93-106	100
Cell culture media (n=8)	95-110	102

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	45.80	148.10	396.90	46.40	141.20	378.00
Standard deviation	3.10	5.90	19.40	2.50	5.90	15.50
CV (%)	6.77	3.98	4.89	5.39	4.18	4.10

Citations

View product citations for antibody STJE0006068 on CiteAb