• Oligodendrocyte. Cells were seeded at a concentration of 5,000 cells/cm2 on tissue culture plastic plates and coverslips and cultured in high glucose DMEM supplemented with 1% Penicillin/Streptomycin, 2 mmol/l L-Glutamine, 1X N1 supplement, 1 µg/ml biotin, 5 ng/ml bFGF, 1 ng/ml PDGF, and 30% B104-conditioned media for 1 day. On the second day, CG4 rat oligodendrocyte progenitor cells were added in a co-culture setting to promote differentiation, using co-culture membrane inserts, and the media were changed every 2 days. The cells were allowed to differentiate for 5 days and were then fixed and stored in PBS for immunofluorescence. Cells were subsequently assessed for expression of the oligodendrocyte markers O2 and NG2.
  • Control medium with recombinant (rec) IL-8 or PDGF-AB/BB was also included.
  • After being subcultured at a concentration of 1 × 106 cells/cm2, BM-MSCs were incubated in Alpha MEM containing 1 mM BME without serum for 24 h. The culture medium was then replaced with Alpha MEM containing 10% FBS and 35 ng/ml at-RA. After three days, the cells were finally transferred to inducer medium containing Alpha MEM, 10% FBS and trophic factors of 5  Mu M FSK, 10 ng/ml bFGF, 5 ng/ml PGF, and 200 ng/ml HG. The cells were cultured for 10 days [
  • Cells in coverslips were starved for 24 h and then pretreated with or without Na2SO3/NaHSO3 at 15  Mu mol/l for 30 min, as well as with PDGF-BB at 50 ng/ml treatment for 24 h for immunofluorescence assay of BrdU incorporation.

Rat PDGFR beta protein (Recombinant) (STJP000592)

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STJP000592

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Rat PDGFR beta-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised 0.2 Mu m filtered PBS solution, pH7.2.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Gene Symbol: Pdgfrb
Gene ID: 24629
Uniprot ID: PGFRB_RAT
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of rat PDGFR beta ncluding a C-terminal 6His tag was expressed in HEK293 cells. NA
Post Translational Modifications N-glycosylated. Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-578, and to a lesser degree, Tyr-580 is important for interaction with SRC. Phosphorylation at Tyr-715 is important for interaction with GRB2. Phosphorylation at Tyr-739 and Tyr-750 is important for interaction with PIK3R1. Phosphorylation at Tyr-750 is important for interaction with NCK1. Phosphorylation at Tyr-770 and Tyr-856 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-856 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1008 is important for interaction with PTPN11. Phosphorylation at Tyr-1008 and Tyr-1020 is important for interaction with PLCG1. Dephosphorylated by PTPRJ at Tyr-750, Tyr-856, Tyr-1008 and Tyr-1020. Dephosphorylated by PTPN2 at Tyr-578 and Tyr-1020.
Function Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Protein Name Platelet-Derived Growth Factor Receptor Beta
Pdgf-R-Beta
Pdgfr-Beta
Beta Platelet-Derived Growth Factor Receptor
Beta-Type Platelet-Derived Growth Factor Receptor
Cd140 Antigen-Like Family Member B
Platelet-Derived Growth Factor Receptor 1
Pdgfr-1
Cd Antigen Cd140b
Database Links Reactome: R-RNO-1257604
Reactome: -RNO-186763
Reactome: -RNO-186797
Reactome: -RNO-5673001
Reactome: -RNO-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle
Lysosome Lumen
After Ligand Binding
The Autophosphorylated Receptor Is Ubiquitinated And Internalized
Leading To Its Degradation
Alternative Protein Names Platelet-Derived Growth Factor Receptor Beta protein
Pdgf-R-Beta protein
Pdgfr-Beta protein
Beta Platelet-Derived Growth Factor Receptor protein
Beta-Type Platelet-Derived Growth Factor Receptor protein
Cd140 Antigen-Like Family Member B protein
Platelet-Derived Growth Factor Receptor 1 protein
Pdgfr-1 protein
Cd Antigen Cd140b protein
Pdgfrb protein
Pdgfr protein
Pdgfr1 protein

Information sourced from Uniprot.org

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