This OX Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma, tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Rat
Sensitivity
6.7pg/mL
Detection Limit
15.6-1000pg/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates or other biological fluids.
Additional Info
Tissue Specificity
Mainly expressed in the brain, with expression in neurons in the dorsal lateral hypothalamic area (at protein level). Produced by a small group of neurons restricted to the lateral and posterior hypothalamus and perifornical areas. Positive neurons project widely throughout the entire neuroaxis. Particularly abundant projections in the cerebral cortex, olfactory bulb, hippocampus, amygdala, septum, diagonal band of Broca, bed nucleus of the stria terminalis, thalamus, anterior and posterior hypothalamus, midbrain, brainstem, and spinal cord. Also expressed in the enteric nervous system and pancreas. In small amount, also detected in the testis.
Post Translational Modifications
Specific enzymatic cleavages at paired basic residues yield the different active peptides.
Function
Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. A modulation effect on luteinizing hormone-releasing hormone (LHRH) secretion also suggests a more minor contribution to the regulation of reproductive function. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.
Protein Name
Hypocretin Neuropeptide PrecursorHypocretinHcrtOrexin PrecursorPrepro-OrexinPreprohypocretin Cleaved Into - Orexin-AHypocretin-1Hcrt1 - Orexin-BHypocretin-2Hcrt2
Rough Endoplasmic ReticulumCytoplasmic VesicleSynapseAssociated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses
