Rat Nphs1 (Nephrin) Sandwich ELISA Kit (STJE0006375)

Name: Rat Nphs1 (Nephrin) Sandwich ELISA Kit (STJE0006375)
SKU: STJE0006375
Availability: InStock

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SPECIFICATIONS

STJE0006375

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Short Description	This Nphs1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Nphs1
Gene ID	64563
Uniprot ID	NPHN_RAT
Specificity	This kit recognizes Rat NPHN in samples. No significant cross-reactivity or interference between Rat NPHN and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylated at Tyr-1204 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1.
Function	Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Protein Name	Nephrin Renal Glomerulus-Specific Cell Adhesion Receptor
Database Links	Reactome: R-RNO-373753
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Located At Podocyte Slit Diaphragm Between Podocyte Foot Processes
Alternative ELISA Names	Nephrin ELISA kit Renal Glomerulus-Specific Cell Adhesion Receptor ELISA kit Nphs1 ELISA kit Nphn ELISA kit
Specificity	This kit recognizes Rat NPHN in samples. No significant cross-reactivity or interference between Rat NPHN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.54	1.27	4.27	0.53	1.29	3.89
Standard deviation	0.03	0.06	0.19	0.03	0.07	0.14
CV (%)	5.56	4.72	4.45	5.66	5.43	3.60