Rat MAG (Myelin-Associated Glycoprotein) Antibody Pair (STJAP00336)
General Information
| Short Description | This MAG antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Mag |
| Gene ID | 29409 |
| Uniprot ID | MAG_RAT |
| Immunogen | Capture immunogen: Recombinant Rat MAG protein.Detection Immunogen: Recombinant Rat MAG protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. Phosphorylated on tyrosine residues. Ubiquitinated, leading to proteasomal degradation. |
| Function | Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury.protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults.this probably depends on binding to gangliosides on the axon cell membrane. Negative regulator of neurite outgrowth.in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth. Inhibits axon outgrowth by binding to RTN4R. Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b. |
| Protein Name | Myelin-Associated Glycoprotein1b236Brain Neuron Cytoplasmic Protein 3Sialic Acid-Binding Ig-Like Lectin 4aSiglec-4a |
| Database Links | Reactome: R-RNO-193634Reactome: -RNO-210991 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Pair Names | Myelin-Associated Glycoprotein antibody pair1b236 antibody pairBrain Neuron Cytoplasmic Protein 3 antibody pairSialic Acid-Binding Ig-Like Lectin 4a antibody pairSiglec-4a antibody pairMag antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat MAG (Myelin-Associated Glycoprotein) Antibody Pair (STJAP00336)
This antibody pair is designed to detect Rat MAG (Myelin-Associated Glycoprotein), corresponding to UniProt accession P07722. Reactivity has been confirmed for rat samples only.
The capture antibody is an unconjugated mouse monoclonal antibody supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4. The detection antibody is a biotin-conjugated goat polyclonal antibody supplied at the same concentration and formulation.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specification.
Both the capture and detection antibodies were raised against recombinant Rat MAG protein. The product details do not specify the immunogen region used for either component.
The capture antibody was purified by Protein A or G affinity chromatography, while the detection antibody was purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.