Rat IL-1 Alpha (Interleukin-1 Alpha) Antibody Pair (STJAP00339)
General Information
| Short Description | This IL-1 Alpha antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Il1a |
| Gene ID | 24493 |
| Uniprot ID | IL1A_RAT |
| Immunogen | Capture immunogen: Recombinant Rat IL-1 Alpha protein.Detection Immunogen: Recombinant Rat IL-1 Alpha protein. |
Additional Info
| Function | Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity. |
| Protein Name | Interleukin-1 AlphaIl-1 Alpha |
| Database Links | Reactome: R-RNO-448706Reactome: -RNO-5620971Reactome: -RNO-9020702 |
| Cellular Localisation | NucleusCytoplasmSecretedThe Lack Of A Specific Hydrophobic Segment In The Precursor Sequence Suggests That Il-1 Is Released By Damaged Cells Or Is Secreted By A Mechanism Differing From That Used For Other Secretory ProteinsThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And SecretionRecruited To Dna Damage Sites And Secreted After Genotoxic Stress |
| Alternative Antibody Pair Names | Interleukin-1 Alpha antibody pairIl-1 Alpha antibody pairIl1a antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat IL-1 Alpha (Interleukin-1 Alpha) Antibody Pair (STJAP00339)
This antibody pair is designed to detect rat IL-1 Alpha (Interleukin-1 Alpha), with reactivity confirmed for rat. Both the capture and detection antibodies were raised against recombinant rat IL-1 Alpha protein, corresponding to UniProt entry P16598.
The capture antibody is an unconjugated mouse monoclonal antibody, and the detection antibody is a biotin-conjugated goat polyclonal antibody. Both components are supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4.
This antibody pair has been validated for use in ELISA. No additional assay formats are specified in the product documentation.
The capture antibody has been purified using Protein A or G, while the detection antibody has been purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.