Rat GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This rat GDNF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Rat |
| Sensitivity | 9.38pg/mL |
| Detection Limit | 15.63~1000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | Gdnf |
| Gene ID | 25453 |
| Uniprot ID | GDNF_RAT |
| Specificity | This kit recognizes Rat GDNF in samples. No significant cross-reactivity or interference between Rat GDNF and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in both the central nervous system (CNS) and in non-CNS tissues, including the kidney, lung, bone, heart, liver, spleen, sciatic nerve and blood. Expressed in brain (at protein level). Localizes at the proximal ligature of the hypoglossal nerve. |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. May also modulate local neuronal effects in distal regions of the motor neuron. Involved in the development of the neural crest. |
| Protein Name | Glial Cell Line-Derived Neurotrophic FactorAstrocyte-Derived Trophic FactorAtf |
| Database Links | Reactome: R-RNO-5673001Reactome: -RNO-8853659 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Glial Cell Line-Derived Neurotrophic Factor CLIA kitAstrocyte-Derived Trophic Factor CLIA kitAtf CLIA kitGdnf CLIA kit |
| Specificity | This kit recognizes Rat GDNF in samples. No significant cross-reactivity or interference between Rat GDNF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-99 | 91 |
| EDTA plasma(n=8) | 86-98 | 93 |
| Cell culture media(n=8) | 94-110 | 101 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 54.07 | 121.99 | 408.12 | 54.44 | 128.92 | 436.94 |
| Standard deviation | 4.46 | 13.91 | 45.22 | 6.07 | 13.25 | 39.41 |
| CV (%) | 8.25 | 11.40 | 11.08 | 11.15 | 10.28 | 9.02 |