Rat E Cadherin (E Cadherin) Antibody Pair (STJAP00772)
General Information
| Short Description | This E Cadherin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R84-3I-2/R15-4F-7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Cdh1 |
| Gene ID | 83502 |
| Uniprot ID | CADH1_RAT |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. N-glycosylation at Asn-641 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-758. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-289 and Thr-513 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. |
| Protein Name | Cadherin-1Epithelial CadherinE-CadherinUvomorulinCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 |
| Database Links | Reactome: R-RNO-1474228Reactome: -RNO-216083Reactome: -RNO-351906Reactome: -RNO-418990 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell MembraneSingle-Pass Type I Membrane ProteinEndosomeGolgi ApparatusTrans-Golgi NetworkCytoplasmDesmosomeColocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial CellsAnchored To Actin Microfilaments Through Association With Alpha-Beta- And Gamma-CateninSequential Proteolysis Induced By Apoptosis Or Calcium InfluxResults In Translocation From Sites Of Cell-Cell Contact To The CytoplasmColocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma MembraneRecruited To Desmosomes At The Initial Assembly Phase And Also Accumulates Progressively At Mature Desmosome Cell-Cell JunctionsLocalizes To Cell-Cell Contacts As Keratinocyte Differentiation Progresses |
| Alternative Antibody Pair Names | Cadherin-1 antibody pairEpithelial Cadherin antibody pairE-Cadherin antibody pairUvomorulin antibody pairCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibody pairCdh1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat E Cadherin (E Cadherin) Antibody Pair (STJAP00772)
This antibody pair is validated for the detection of rat E-Cadherin (UniProt Q9R0T4) and has confirmed reactivity against rat samples only. It is intended for research use only and is not validated for detection in other species.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs (clones R84-3I-2 and R15-4F-7) at 1 mg/mL, formulated in 0.01M PBS at pH 7.2. Neither antibody carries an enzyme or fluorescent conjugate, so the user is responsible for labeling the detection antibody as required for their assay setup.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Optimal working concentrations are determined by the end user and are not provided in the product specifications. Titration experiments are recommended to establish the appropriate concentrations for each antibody within the user's specific assay conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.