Rat DRD3 (Dopamine Receptor D3) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This rat DRD3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Rat |
| Sensitivity | 37.5pg/mL |
| Detection Limit | 62.5~4000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | Drd3 |
| Gene ID | 29238 |
| Uniprot ID | DRD3_RAT |
| Specificity | This kit recognizes Rat DRD3 in samples. No significant cross-reactivity or interference between Rat DRD3 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Mainly in limbic areas of brain. |
| Post Translational Modifications | Phosphorylated by GRK4. Palmitoylated. |
| Function | Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. |
| Protein Name | D(3 Dopamine ReceptorDopamine D3 Receptor |
| Database Links | Reactome: R-RNO-390651Reactome: -RNO-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | D(3 Dopamine Receptor CLIA kitDopamine D3 Receptor CLIA kitDrd3 CLIA kit |
| Specificity | This kit recognizes Rat DRD3 in samples. No significant cross-reactivity or interference between Rat DRD3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-112 | 103 |
| EDTA plasma(n=8) | 88-101 | 95 |
| Cell culture media(n=8) | 99-115 | 107 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 219.85 | 510.66 | 1716.98 | 216.22 | 557.03 | 1623.01 |
| Standard deviation | 23.28 | 56.27 | 174.79 | 24.71 | 58.88 | 148.99 |
| CV (%) | 10.59 | 11.02 | 10.18 | 11.43 | 10.57 | 9.18 |