rat Cyclophilin F/PPIF protein (Recombinant) (His-Tag) (STJP019302)
SPECIFICATIONS
HostE.coli
ImmunogenRat
General Information
| Short Description | Recombinant-rat Cyclophilin F/PPIF-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 1, 300nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. |
Target Information
| Gene Symbol | Ppif |
| Gene ID | 282819 |
| Uniprot ID | PPIF_RAT |
| Accession Number | NP_758443 |
| Immunogen | Rat |
| Immunogen Region | 30-206aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS |
Additional Info
| Post Translational Modifications | Acetylated at Lys-166.deacetylated at Lys-166 by SIRT3. |
| Function | PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis.the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase F - MitochondrialPpiase FCyclophilin DCyp-DCypdCyclophilin FRotamase F |
| Database Links | |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Protein Names | Peptidyl-Prolyl Cis-Trans Isomerase F - Mitochondrial proteinPpiase F proteinCyclophilin D proteinCyp-D proteinCypd proteinCyclophilin F proteinRotamase F proteinPpif protein |
Information sourced from Uniprot.org