Rat CRT (Calreticulin) CLIA Kit for Quantitative Detection

Name: Rat CRT (Calreticulin) CLIA Kit for Quantitative Detection
SKU: STJC0001573
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0001573

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General Information

Short Description	This rat CRT kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Rat
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Target Information

Gene Symbol	Calr
Gene ID	64202
Uniprot ID	CALR_RAT
Specificity	This kit recognizes Rat CRT in samples. No significant cross-reactivity or interference between Rat CRT and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Tissue Specificity	Predentin and odontoblast.
Function	Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.
Protein Name	Calreticulin Calbp Crp55 Calcium-Binding Protein 3 Cabp3 Calregulin Endoplasmic Reticulum Resident Protein 60 Erp60 Hacbp
Database Links	Reactome: R-RNO-1236974 Reactome: -RNO-3000480 Reactome: -RNO-901042 Reactome: -RNO-983170
Cellular Localisation	Endoplasmic Reticulum Lumen Cytoplasm Cytosol Secreted Extracellular Space Extracellular Matrix Cell Surface Sarcoplasmic Reticulum Lumen Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Cytolytic Granule Also Found In Cell Surface (T Cells) Cytosol And Extracellular Matrix During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex In Cortical Granules Of Non-Activated Oocytes Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation
Alternative CLIA Names	Calreticulin CLIA kit Calbp CLIA kit Crp55 CLIA kit Calcium-Binding Protein 3 CLIA kit Cabp3 CLIA kit Calregulin CLIA kit Endoplasmic Reticulum Resident Protein 60 CLIA kit Erp60 CLIA kit Hacbp CLIA kit Calr CLIA kit
Specificity	This kit recognizes Rat CRT in samples. No significant cross-reactivity or interference between Rat CRT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-101	96
EDTA plasma(n=8)	89-103	95
Cell culture media(n=8)	91-104	97

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	49.66	111.14	338.03	49.52	106.52	345.16
Standard deviation	4.48	10.17	32.89	5.46	11.65	35.76
CV (%)	9.02	9.15	9.73	11.03	10.94	10.36

Citations

View product citations for antibody STJC0001573 on CiteAb