• Human peripheral blood was collected (30 ml) from adult healthy donors after obtaining the IRB approval from the Ohio State University Medical Center and obtaining written consents from donors. The ethic committee has also approved the procedure and records are saved in the laboratory logbook. Freshly collected blood was processed to isolate peripheral blood mononuclear cells (PBMC) following the similar protocol published earlier
  • Rat vascular smooth muscle cells (VSMCs) were isolated by enzymatic digestion of thoracic aortic media from male Sprague-Dawley rats (250–300g, obtained from Tongji Medical College, HUST) by the method of
  • Functional assays for the measurement of degranulation (CD107a/b) were performed as described in
  • To assess the impact of MSCs and SB623 cells on the maturation of dendritic cells, monocyte-derived dendritic cells were generated in the presence of GM-CSF and IL-4. On Day 5, human TNF-Alpha (10 ng/ml) was added to each well with or without MSCs or SB623 cells. As previous studies confirmed a role of cyclosporin A in hindering dendritic cell maturation [

Rat CD89 (FCAR) protein (Recombinant) (STJP000486)

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STJP000486

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Rat CD89 (FCAR)-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: 0.2 Mu m filtered PBS solution, pH7.2.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by immobilized recombinant CD89 (10ug/ml) ability to bind human IgA in functional ELISA. NA
Gene Symbol: FCAR
Gene ID: 2204
Uniprot ID: FCAR_HUMAN
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of Rat CD89including a C-terminal 6His tag was expressed in HEK293 cells. NA
Tissue Specificity Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages.however only one isoform is expressed at alveolar macrophages surfaces.
Function Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production.
Protein Name Immunoglobulin Alpha Fc Receptor
Iga Fc Receptor
Cd Antigen Cd89
Database Links Reactome: R-HSA-6798695
Cellular Localisation Isoform A
1: Cell Membrane
Single-Pass Type I Membrane Protein
2: Cell Membrane
3: Cell Membrane
Isoform B: Secreted
Isoform B-Delta-S2: Secreted
Alternative Protein Names Immunoglobulin Alpha Fc Receptor protein
Iga Fc Receptor protein
Cd Antigen Cd89 protein
FCAR protein
CD89 protein

Information sourced from Uniprot.org

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