• Human monocytes were obtained from leukocyte-enriched buffy coats obtained from healthy volunteer blood donors drawn at the of in accordance with the written approval of the Director of the and the and Ethics Committee of the University of, Medical and Health Science. Written informed consent was obtained from the donors prior to blood donation, and their data were processed and stored according to the principles expressed in the Declaration of Helsinki. Human monocytes were isolated as described previously
  • (f) Sorted IMCs were cultured with control medium (Med) , G-CSF (50 ng ml−1) , M-CSF (10 ng ml−1) , GM-CSF (10 ng ml−1) , or IL-5 (10 ng ml−1) for 2–4 days, and their absolute numbers were counted on the indicated days.
  • To determine whether circulating factors may play a role in osteoclastogenesis after long-term MTX treatments, in vitro osteoclast formation assay was performed to examine whether plasma derived from treated rats could induce osteoclast formation from bone marrow cells of normal rats. Briefly, non-adherent hematopoietic cells isolated from normal rats were plated in 96-well trays at the density of 3×105 cells/well in triplicate, and cultured overnight in Alpha-MEM media containing 50 µg/ml Pen/Strep, 15 mM HEPES, 10 ng/ml macrophage-colony stimulating factor (M-CSF) , and 10% plasma from control rats or treated rats. Cells were then fed the next day with similar plasma-containing media excluding M-CSF and any other exogenous osteoclastogenic factors except RANKL for the positive control (M-CSF+RANKL). To determine whether IL-1 Beta plays a role in plasma-induced osteoclast formation, a neutralizing antibody against IL-1 Beta at two different concentrations (0. 04 and 0. 08 ng/ml) was added to the plasma-containing media as described +…
  • at CCL5, CCL7, CCL20 were from& and CXCL2 from.

Rat CD68 protein (Recombinant) (STJP000619)

SKU:
STJP000619

£228.50 - £2,057.50
Shipping:
Free Shipping
Current Stock:
Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Rat CD68-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised 0.2 Mu m filtered PBS solution, pH7.2.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of Rat CD68 (macrosialin) including a C terminal His tag was expressed in HEK293 cells. NA
Background Recombinant Rat CD68 (macrosialin) is a monomer protein consisting of 290 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance