Rat Afp (Alpha-fetoprotein) Sandwich ELISA Kit (STJE0005532)

Name: Rat Afp (Alpha-fetoprotein) Sandwich ELISA Kit (STJE0005532)
SKU: STJE0005532
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0005532

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Short Description	This Afp Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Afp
Uniprot ID	FETA_RAT
Specificity	This kit recognizes Rat Alpha FP in samples. No significant cross-reactivity or interference between Rat Alpha FP and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Sulfated.
Function	Binds estrogens, fatty acids and metals.
Protein Name	Alpha-Fetoprotein Alpha-1-Fetoprotein Alpha-Fetoglobulin
Cellular Localisation	Isoform 1: Secreted Isoform 2: Cytoplasm
Alternative ELISA Names	Alpha-Fetoprotein ELISA kit Alpha-1-Fetoprotein ELISA kit Alpha-Fetoglobulin ELISA kit Afp ELISA kit
Specificity	This kit recognizes Rat αFP in samples. No significant cross-reactivity or interference between Rat αFP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.10	3.00	9.30	1.00	3.30	8.50
Standard deviation	0.10	0.20	0.40	0.10	0.10	0.40
CV (%)	9.09	6.67	4.30	10.00	3.03	4.71