Monoclonal Antibody | Primary Antibodies | St John's Laboratory

Anti-Phospho-CREB1-Ser-133 antibody [M415] (STJA0005351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€399.38

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CREB (Phospho-Ser133) antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-Phospho-CREB1-Ser133 antibody (26-140 aa) [R07-3C4] (STJA0011169)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

€308.13

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Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (STJ11102643) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody [S3MR] (STJ11102643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from 293T cells, using Phospho-Beta-Catenin-S33/S37 Rabbit mAb (STJ11102571) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CTNNB1-S33/S37 antibody [S1MR] (STJ11102571)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Beta-Catenin-S552 antibody (STJ11105815) at 1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CTNNB1-S552 antibody [S5815RM] (STJ11105815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Anti-Phospho-CTNND1-Tyr-228 antibody [M356] (STJA0005311)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€488.13

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Anti-Phospho-DAG1-Tyr-892 antibody [M117] (STJA0005378)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€456.88

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Western blot analysis of various lysates, using Phospho-DRP1-S616 Rabbit monoclonal antibody (STJ11105838) at 1:10000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:100000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-DNM1L-S616 antibody [S5838RM] (STJ11105838)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Anti-Phospho-DPYSL2-Thr-555 antibody [M539] (STJA0005361)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€456.88

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Anti-Phospho-EGFR-Tyr-1101 antibody [M199] (STJA0005398)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€488.13

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Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1068 Rabbit mAb (STJ11102550) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-EGFR-Y1068 antibody [S0MR] (STJ11102550)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of lysates from BxPC-3 cells, using Phospho-EGFR-Y1068 Rabbit mAb (STJ11102583) at 1:1000 dilution. BxPC-3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-EGFR-Y1068 antibody [S3MR] (STJ11102583)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from BxPC-3 cells, using Phospho-EGFR-Y1173 Rabbit mAb (STJ11102582) at 1:1000 dilution. BxPC-3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-EGFR-Y1173 antibody [S2MR] (STJ11102582)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of A-431 cells, using Phospho-EGFR (Y845) Rabbit monoclonal antibody (STJ11105880) at 1:800 dilution. A-431 cells were treated by EGF (200 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EGFR-Y845 antibody [S5880RM] (STJ11105880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (A4869) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-EIF2AK2-T446 antibody [S1MR] (STJ11102631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Immunohistochemistry analysis of paraffin-embedded mouse liver using Phospho-PERK-T982 Rabbit monoclonal antibody (STJ11105876) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-EIF2AK3-T982 antibody [S5876RM] (STJ11105876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of various lysates, using Phospho-GCN2-T899 antibody (STJ11105840) at1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF2AK4-T899 antibody [S5840RM] (STJ11105840)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of various lysates using Phospho-eIF2 Alpha-S51 Rabbit mAb (STJ11102562) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-EIF2S1-S51 antibody [S2MR] (STJ11102562)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-eIF2α (Phospho-Ser51) (PTR1316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 40kDaObserved band size: 40, 90kDa

Anti-Phospho-EIF2S1-Ser51 antibody (1-100) [PTR1316] (STJA0006271)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells, using Phospho-eIF4E-S209 Rabbit mAb (STJ11105791) at 1:1000 dilution. HeLa cells were treated by 20% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-EIF4E-S209 antibody [S5791RM] (STJ11105791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€183.13 - €803.13

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Western blot analysis of extracts of various cell lines, using Phospho-eIF4EBP1-S65 antibody (STJ11105814) at1:20000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-EIF4EBP1-S65 antibody [S5814RM] (STJ11105814)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of NIH/3T3, using Phospho-EIF4EBP1-S65 antibody (STJ11105846) at 1:20000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-S65 antibody [S5846RM] (STJ11105846)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37 Rabbit monoclonal antibody (STJ11105858) at 1:1000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-EIF4EBP1-T37 antibody [S5858RM] (STJ11105858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€183.13 - €803.13

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Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37/T46 Rabbit monoclonal antibody (STJ11105857) at 1:7000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-T37/T46 antibody [S5857RM] (STJ11105857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€183.13 - €803.13

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Western blot analysis of extracts of NIH/3T3, using Phospho-eIF4EBP1-T70 antibody (STJ11105824) at1:5000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-T70 antibody [S5824RM] (STJ11105824)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of lysates from A-431 cells, using Phospho-HER2/ErbB2-Y1139 (STJ11103785) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ErbB2-Y1139 antibody [S5MR] (STJ11103785)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of A-431, using Phospho-ErbB2 (Y1221 + Y1222) Rabbit monoclonal antibody (STJ11105879) at 1:1000 dilution. A-431 cells were treated by EGF (200 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-ERBB2-Y1221/Y1222 antibody [S5879RM] (STJ11105879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (STJ11102622) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ERBB3-Y1222 antibody [S2MR] (STJ11102622)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of extracts from MCF7 cells, using Phospho-ErbB3/HER3-Y1289 Rabbit monoclonal antibody (STJ11105873) at 1:1000 dilution. MCF7 cells were treated by neuregulin at 37 °C for 15 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-Phospho-ERBB3-Y1289 antibody [S5873RM] (STJ11105873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200

Anti-Phospho-ERK-Y222/205 antibody (140-220 aa) [4G3] (STJ97738)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb (201245, 1:500 diluted, upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246, 1:500 diluted, lower).

Anti-Phospho-ERK1/2-Thr202/Tyr204 antibody [2E8-2F6-C12] (STJ99286)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

€180.00 - €435.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ERK1/ERK2-T202/T185 antibody [S6MR] (STJ11102556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ESR Alpha-S118 Rabbit mAb (STJ11102593) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ESR1-S118 antibody [S3MR] (STJ11102593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€183.13 - €803.13

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Anti-Phospho-ESR1-Tyr-537 antibody [M545] (STJA0005416)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€456.88

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Anti-Phospho-FOXO3-S253 antibody [S5793RM] (STJ11105793)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of Phospho-FOXO3A (Ser253) in 3T3 lysates using Phospho-FOXO3A (Ser253) antibody.

Anti-Phospho-FOXO3-Ser253 antibody (248-260 aa) [R06-2G6] (STJA0011330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€308.13

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Anti-Phospho-FSCN1-Ser-39 antibody [M315] (STJA0005419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat

€456.88

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Western blot analysis of various lysates using Phospho-GSK3 Beta-S9 Mouse mAb (STJ11107699) at 1:1000 dilution. HeLa NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GSK3 Beta-S9 antibody [SMM] (STJ11107699)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Anti-Phospho-GSK3A/B-Tyr-216 antibody [M132] (STJA0005434)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€456.88

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Western blot analysis of extracts of NIH/3T3, using Phospho-GSK3 Alpha-S21+GSK3 Beta-S9 antibody (STJ11105830) at 1:800 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-GSK3alpha + GSK3Beta-S21-S9 antibody [S5830RM] (STJ11105830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€183.13 - €803.13

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using GSK3β mouse monoclonal antibody diluted at 1:200.

Anti-Phospho-GSK3B antibody [6H6] (STJ196700)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€217.50 - €315.00

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Anti-Phospho-GSK3B antibody [8D11] (STJ196701)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

€217.50 - €315.00

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Western blot analysis of various lysates using Phospho-GSK3 Beta-S9 Rabbit mAb (STJ11102623) at 1:1000 dilution incubated overnight at 4 °C. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-GSK3B-S9 antibody [S3MR] (STJ11102623)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit mAb (STJ11105699) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GYS1-S641 antibody [S6MR] (STJ11102626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

€180.00 - €435.00

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Western blot analysis of extracts of Jurkat cells, using Phospho-HDAC4-S632 antibody (STJ11105833) at1:2000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-HDAC4-S632 antibody [S5833RM] (STJ11105833)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of various lysates using Phospho-Histone H1. 3-T17/Histone H1. 4-T17 Rabbit mAb (STJ11102629) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H1.3/Histone H1.4-T17/T17 antibody [S9MR] (STJ11102629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Monoclonal Antibodies