Anti-CD43 antibody [DF-1] (STJ16101856)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2k
ImmunogenKG1 cells (myoblast cell line)
General Information
| Short Description | Mouse monoclonal anti-CD43 for use in IHC-P and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | DF-1 |
| Isotype | IgG2k |
| Conjugation | Unconjugated |
| Purification | Concentrate |
| Dilution Range | IHC-P 1:100-1:200WB 1:100-1:200 |
| Formulation | Bioreactor Concentrate with 0.05% Azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
| Immunogen | KG1 cells (myoblast cell line) |
Additional Info
| Background | The anti-CD43 antibody recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation) , identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So, a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining Membranous. |
Information sourced from Uniprot.org