Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CDK2 rabbit monoclonal antibody (STJ11101225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK2 antibody (199-298) [S5MR] (STJ11101225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer using [KO Validated] CDK2 Rabbit polyclonal antibody (STJ113540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK2 antibody (200-298) (STJ113540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (STJ119973) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-CDK2 antibody (200-298) (STJ119973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Cdk2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK2 antibody (231-280 aa) (STJ92197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CDK2 antibody [1C4] (STJA0032600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Monkey/Mouse/Rat

£224.50 - £308.50

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Anti-Cdk2 antibody [5B12D1] (STJ16103574)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC/WB

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-Cdk2 antibody [6D5B5] (STJ16103575)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-CDK2 antibody [PT0233R] (STJA0039631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK2 antibody [R04-1D5] (STJA0033786)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£140.50 - £308.50

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Western blot analysis of CDK2 at dilution of 1:1000

Anti-CDK2 antibody [SM5078] (STJA0005078)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunofluorescence analysis of HUVEC cells, using CCRK Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CDK20 antibody (31-80 aa) (STJ92076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit polyclonal antibody (STJ11100861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDK2AP1 antibody (1-115) (STJ11100861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates using CDK2AP1 Rabbit polyclonal antibody (STJ11104468) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CDK2AP1 antibody (1-115) (STJ11104468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using DOC-1 Polyclonal Antibody diluted at 1：500

Anti-CDK2AP1 antibody (51-100 aa) (STJ92754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CDK3 antibody (10-90 aa) (STJ190276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-CDK4 antibody (1-303aa) [AT6D10] (STJA0041666)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£219.50 - £332.50

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Immunofluorescence analysis of Hela cell. 1, Cdk4 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDK4 antibody (1-50 aa) (STJ96563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit pAb (STJ23055) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CDK4 antibody (200-303) (STJ23055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-CDK4 antibody (204-303 aa) [SRM5126] (STJ11106945)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.

Anti-CDK4 antibody (204-303) (STJ11101180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using CDK4 rabbit monoclonal antibody (STJ11101279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK4 antibody (204-303) [S9MR] (STJ11101279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£143.50 - £618.50

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Western blot detection of CDK4 in Hela, Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

Anti-CDK4 antibody [3F9-B12-C8] (STJ99110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Anti-CDK4 antibody [3F9-B12-C8] (STJA0032384)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Cdk4 antibody [DCS-31] (STJ16100131)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IF/WB/FC/IP

Reactivity :

Human/Mouse/Rat

£1,025.50

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Anti-CDK4 antibody [PT0399R] (STJA0039565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK4 antibody [R04-4I5] (STJA0034157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-CDK4 antibody [R04-8I5] (STJA0034156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-CDK4 antibody [R07-4G7] (STJA0034158)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-CDK4 antibody [SQab1864] (STJ16102878)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ICC/IF/IHC-P/IP/WB

Reactivity :

Human

£708.50

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Anti-CDK4 antibody [SRM13318] (STJ11108048)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunoprecipitation analysis of 900 Mu g extracts of A-549 cells using 3 Mu g CDK5 antibody (STJ28297). Western blot was performed from the immunoprecipitate using CDK5 antibody (STJ28297) at a dilution of 1:1000.

Anti-CDK5 antibody (1-292) (STJ28297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HepG2 cells using Cdk5 Polyclonal Antibody diluted at 1：500

Anti-CDK5 antibody (1-50 aa) (STJ92200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells, using [KO Validated] CDK5 Rabbit polyclonal antibody (STJ11100053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK5 antibody (170-289) (STJ11100053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of Hela, using CDK5 rabbit monoclonal antibody (STJ11101567) at 1:1000 dilutionSecondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDK5 antibody (193-292) [S7MR] (STJ11101567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells, using [KO Validated] CDK5 Rabbit monoclonal antibody (STJ11105132) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK5 antibody (92-289 aa) [S5132RM] (STJ11105132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Cdk5 antibody (Full-Length) [DC17+DC34] (STJA0041114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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Western blot detection of CDK5 (N-terminus) in 3T3, Hela, PC-12, COS7, Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.

Anti-CDK5 antibody (N-Term) [2E8-F9-B7-C11] (STJ99113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot of rat striatal lysate showing specific immunolabeling of the ~28 kDa Cdk5 protein.

Anti-CDK5 antibody [1H3] (STJA0003592)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£488.50

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Anti-CDK5 antibody [2E8-F9-B7-C11] (STJA0032387)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat/Monkey

£133.50 - £281.50

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Anti-CDK5 antibody [2H6] (STJA0032601)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Western blot analysis of1) Hela Cell, 2) Jurkat Cell, 3) Mouse Brain Tissue, 4) Rat Brain Tissue lysate using mouse monoclonal antibody diluted at 1:2,000.

Anti-CDK5 antibody [3D1] (STJ196638)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-CDK5 antibody [DC17+DC34] (STJA0005342)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-CDK5 antibody [PT1229R] (STJA0040176)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK5 antibody [R08-9I3] (STJA0035917)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-CDK5 antibody [SRM14897] (STJ11109627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of Hela cell. 1, p35 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK5R1 antibody (11-60 aa) (STJ94875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using CDK5R1 Rabbit monoclonal antibody (STJ11105105) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDK5R1 antibody (200-300 aa) [S5105RM] (STJ11105105)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of SH-SY5Y cells using CDK5R1 antibody (STJ116708) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDK5R1 antibody (99-307) (STJ116708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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