Anti-CDK4 antibody [SQab1864] (STJ16102878)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
ImmunogenSynthetic peptide around the C-terminus of CDK4.
General Information
| Short Description | Rabbit monoclonal anti-CDK4 for use in FC, ICC, IF, IHC-P, IP and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/ICC/IF/IHC-P/IP/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SQab1864 |
| Conjugation | Unconjugated |
| Purification | Purification with Protein A. |
| Formulation | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Storage Instruction | Store at-20°C for up to 1 year. Minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CDK4 |
| Gene ID | 1019 |
| Uniprot ID | CDK4_HUMAN |
| Immunogen | Synthetic peptide around the C-terminus of CDK4. |
Additional Info
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Protein Name | Cyclin-Dependent Kinase 4Cell Division Protein Kinase 4Psk-J3 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-3214858Reactome: R-HSA-381340Reactome: R-HSA-69231Reactome: R-HSA-75815Reactome: R-HSA-8849470Reactome: R-HSA-8878166Reactome: R-HSA-912446Reactome: R-HSA-9616222Reactome: R-HSA-9630791Reactome: R-HSA-9630794Reactome: R-HSA-9632697Reactome: R-HSA-9632700Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | CytoplasmNucleusNucleus MembraneCytoplasmic When Non-ComplexedForms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) PhaseThe Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S PhaseAlso Present In Nucleoli And Heterochromatin LumpsColocalizes With Rb1 After Release Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 4 antibodyAnti-Cell Division Protein Kinase 4 antibodyAnti-Psk-J3 antibodyAnti-CDK4 antibody |
Information sourced from Uniprot.org