Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of MCF-7 cells using DDB2 antibody (STJ11104312). Blue: DAPI for nuclear staining.

Anti-DDB2 antibody (1-315) (STJ11104312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using DDB2 antibody (STJ23354) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDB2 antibody (1-315) (STJ23354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using DDC antibody (STJ23355) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DDC antibody (1-137) (STJ23355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DDDDK-Tag using Rabbit anti DDDDK-Tag pAb (STJ11103696) at 1:10000 dilution. <br/>Secondary antibody: (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-DDDDK-Tag antibody (STJ11103696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP

Reactivity :

Species independent

€200.63 - €375.63

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Mouse anti DDDDK-Tag using Mouse anti DDDDK-Tag mAb (STJ11103697) at 1:20000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 20 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit ( Exposure time: 45s.

Anti-DDDDK-Tag antibody [S7MM] (STJ11103697)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/IP/FC/ELISA

Reactivity :

Species independent

€200.63 - €375.63

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Western blot analysis of lysates from C2C12, using DDIT3/CHOP rabbit polyclonal antibody (STJ116305) at 1:1000 dilution. C2C12 cells were treated by tunicamycin (2 Mu g/ml) for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DDIT3 antibody (1-169) (STJ116305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using DDIT3/CHOP Rabbit polyclonal antibody (STJ23356) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DDIT3 antibody (1-169) (STJ23356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.

Anti-DDIT3 antibody (10-90 aa) [1E1] (STJ97775)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Calnexin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-DDIT3 antibody (10-90 aa) [2B1] (STJ97774)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using CHOP Polyclonal Antibody diluted at 1：500

Anti-DDIT3 antibody (15-64 aa) (STJ92277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200

Anti-DDR2 antibody (31-80 aa) (STJ98753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/IHC/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis using DDR2 monoclonal antibody against truncated DDR2 recombinant protein.

Anti-DDR2 antibody [3B11E4] (STJ97997)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200

Anti-DDT antibody (50-90 aa) (STJ98703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 293F, using DDX1 rabbit polyclonal antibody (STJ28658) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DDX1 antibody (441-740) (STJ28658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using DDX19B Polyclonal Antibody

Anti-DDX19B antibody (1-50 aa) (STJ92674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells using DDX20 antibody (STJ28380). Blue: DAPI for nuclear staining.

Anti-DDX20 antibody (25-280) (STJ28380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded mouse testis using DDX21 antibody (STJ117020) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DDX21 antibody (1-180) (STJ117020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunocytochemistry analysis of DDX21 (green) in 293T using DDX21 antibody, and DAPI (blue).

Anti-DDX21 antibody (248-260 aa) [R04-4J2] (STJA0011226)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

€308.13

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Immunohistochemistry analysis of paraffin-embedded human brain, using DDX24 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-DDX24 antibody (41-90 aa) (STJ92675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of U-2 OS cells using DDX24 Rabbit polyclonal antibody (STJ117676) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DDX24 antibody (510-859) (STJ117676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-DDX3 antibody (100-200 aa) [SRM] (STJ11107047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of L929 cells using DDX31 Rabbit polyclonal antibody (STJ118337) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DDX31 antibody (652-851) (STJ118337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using DDX39A Rabbit pAb (STJ110264) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DDX39A antibody (1-100) (STJ110264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using UAP56/DDX39B Rabbit pAb (STJ110654) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-DDX39B antibody (319-428) (STJ110654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-DDX3X antibody (100-200) (STJ11105653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using DDX3X antibody (STJ27604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX3X antibody (17-33) (STJ27604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunocytochemistry analysis of DDX3 (6G8) in HeLa using DDX3 antibody.

Anti-DDX3X antibody (22-178 aa) [6G8-F4-E3] (STJA0012848)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP/ChIP

Reactivity :

Human/Rat/Mouse/Monkey

€330.63

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Western blot analysis of various cells using DDX3 Polyclonal Antibody

Anti-DDX3X antibody (466-515 aa) (STJ92676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U-2 OS cells using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DDX3Y antibody (1-210) (STJ113303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DDX3Y Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-DDX3Y antibody (41-90 aa) (STJ92678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of mouse testis, using DDX4 antibody (STJ118072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DDX4 antibody (635-724) (STJ118072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis using DDX4 monoclonal antibody against DDX4-hIgGFc transfected HEK293 cell lysate.

Anti-DDX4 antibody [2F9H5] (STJ97998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g DDX41 antibody (STJ28659). Western blot was performed from the immunoprecipitate using DDX41 antibody (STJ28659) at a dilution of 1:1000.

Anti-DDX41 antibody (1-300) (STJ28659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using p68 RNA Helicase Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DDX5 antibody (565-614 aa) (STJ94913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunocytochemistry analysis of DDX5 (green) in Hela using DDX5 antibody, and DAPI (blue)

Anti-DDX5 antibody (9-21 aa) [R04-2K2] (STJA0011227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€308.13

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Western blot analysis of various lysates using DDX50 Rabbit pAb (STJ113576) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DDX50 antibody (1-135) (STJ113576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using DDX51 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DDX51 antibody (617-666 aa) (STJ92679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using DDX52 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DDX52 antibody (550-599 aa) (STJ92680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using DDX55 Polyclonal Antibody diluted at 1：500

Anti-DDX55 antibody (91-140 aa) (STJ92682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of U20S cells using Rig-I/DDX58 antibody (STJ115369) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DDX58 antibody (726-925) (STJ115369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ73481 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-DDX58 antibody (C-Term) (STJ73481)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse

€390.63

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Western blot analysis of various lysates using DDX6 Rabbit mAb (STJ11102512) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-DDX6 antibody (101-200) [S2MR] (STJ11102512)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of extracts of various cell lines, using DDX6 antibody (STJ118705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX6 antibody (414-533) (STJ118705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-DEC-205/CD205 antibody [NLDC145] {Alexa-fluor-546} (STJ16102647)

Clonality :

Monoclonal

Host :

Rat

Applications :

FACS/IHC-F/IF

Reactivity :

Mouse

€963.13

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Anti-DEC205/CD 205 (NLDC145) antibody [NLDC145] (STJ16102000)

Clonality :

Monoclonal

Host :

Rat

Applications :

FACS/IHC-F/IF

Reactivity :

Mouse

€795.63

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Anti-DEC205/CD 205 (NLDC145) antibody [NLDC145] {Alexa-fluor-488} (STJ16101998)

Clonality :

Monoclonal

Host :

Rat

Applications :

FACS/IF

Reactivity :

Mouse

€963.13

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Anti-Dectin-1 antibody (66-247 aa) [SRM] (STJ11107319)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of Jurkat cells using DEFA1 Rabbit polyclonal antibody (STJ28977) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DEFA1 antibody (1-94) (STJ28977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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