Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis using HXK II monoclonal antibody against Jurkat (1) , HeLa (2) and HEK293 (3) cell lysate.

Anti-HK2 antibody [3D3] (STJ98152)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis of various lysates using HK3 Rabbit pAb (STJ110726) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HK3 antibody (1-270) (STJ110726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Jurkat cells, treated with insulin 0.01U/ml 15', using Hexokinase-3 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-HK3 antibody (811-860 aa) (STJ93631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-HKCR antibody [109B8.01] {Alexa-fluor-488} (STJ16102228)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human

£820.50

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Anti-HKCR antibody [109B8.01] {Alexa-fluor-546} (STJ16102229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human

£820.50

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Anti-HLA A antibody [R03-2H8] (STJA0035826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-HLA A antibody [R05-5H7] (STJA0034418)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-HLA B7 antibody [SRM13635] (STJ11108365)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-HLA DPB1 antibody [R09-6H3] (STJA0036348)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-HLA DR antibody [R02-6G7] (STJA0036055)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-HLA DRB1 antibody [R02-4K1] (STJA0036664)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates using MHC class I (HLA-A/B) Rabbit mAb (STJ11102369) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-HLA-A/HLA-B antibody (200-300) [S9MR] (STJ11102369)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-HLA-A02:01-peptide (EBV-LMP2A: CLGGLLTMV) Complex antibody [MAB243] (STJA0032136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FCM/ICC/IF/IHC/WB

Reactivity :

Human

£367.50

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Anti-HLA-B27 antibody [EP-4] (STJ16103721)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IF

Reactivity :

Human

£387.50

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Anti-HLA-D antibody [7.5.10.1] (STJ16100014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF

Reactivity :

Human

£225.50

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-HLA-DMB antibody (40-100 aa) (STJ98988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of various cells using HLA-DO Alpha Polyclonal Antibody

Anti-HLA-DOA antibody (71-120 aa) (STJ93542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Confocal imaging of paraffin-embedded Mouse spleen tissue using HLA-DPB1 Rabbit mAb (STJ11107363, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-HLA-DPB1 antibody (1-100 aa) [SRM5449] (STJ11107363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 Rabbit polyclonal antibody (STJ119591) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HLA-DPB1 antibody (40-100) (STJ119591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HLA-DPB1 antibody [SRM14844] (STJ11109574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Anti-HLA-DQA1 antibody (21-70 aa) (STJ98824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of PC12 cells using HLA-DQB1/2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HLA-DQB1/2 antibody (131-180 aa) (STJ96857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Anti-HLA-DR antibody (100-200 aa) [SRM5334] (STJ11107240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£143.50 - £618.50

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Anti-HLA-DR antibody [PT0377R] (STJA0039581)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-HLA-DR antibody [SRM13513] (STJ11108243)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-HLA-DRw52 antibody [7.3.19.1] (STJ16100015)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF

Reactivity :

Human

£225.50

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Anti-HLA-G antibody [SRM14749] (STJ11109479)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-HLTF antibody [R03-1E4] (STJA0034420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-HLTF antibody [R07-6M2] (STJA0037172)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of various cells using HLX1 Polyclonal Antibody diluted at 1：1000

Anti-HLX antibody (321-370 aa) (STJ93546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of MCF7 cells, using GPR109 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HM74 antibody (285-334 aa) (STJ93547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of A-549 cells using HMG20A antibody (STJ29425).

Anti-HMG20A antibody (1-347) (STJ29425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using BRAF35 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-HMG20B antibody (1-50 aa) (STJ91880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-HMG4 antibody [7G13] (STJ16103426)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC/ICC/WB

Reactivity :

Human

£746.50

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Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody (STJ11102046) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-HMGA1 antibody (1-107) [S6MR] (STJ11102046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HMGA1 antibody (20-60 aa) (STJ196532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HMGA1 antibody [PT1205R] (STJA0040185)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-HMGA1 antibody [R01-9D3] (STJA0034421)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-HMGA1 antibody [R05-4S2] (STJA0036789)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-HMGA2 antibody [PT1165R] (STJA0040218)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£150.00 - £540.00

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Anti-HMGA2 antibody [SRM14744] (STJ11109474)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (STJ11101678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HMGB1 antibody (100-200) [S8MR] (STJ11101678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, HMG-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HMGB1 antibody (N-Term) (STJ96867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HMGB1 antibody [DPH11] (STJA0019032)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/FuncS/IF/WB

Reactivity :

Human/Mouse/Rat/Bovine/Dog/Horse/Pig

£209.50 - £1,333.50

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Anti-HMGB1 antibody [PT0499R] (STJA0039474)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-HMGB1 antibody [R06-2K1] (STJA0035757)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g HMGB2 Rabbit mAb (STJ11102444) , along with a Goat Anti-Rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1) , as shown in figure.

Anti-HMGB2 antibody (1-100) [S4MR] (STJ11102444)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates using HMGB2 Rabbit polyclonal antibody (STJ119486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMGB2 antibody (100-200) (STJ119486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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