Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, IL-1 Beta Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL1B antibody (181-230 aa) (STJ96566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using IL-1 Beta monoclonal antibody against HEK293 (1) and IL-1 Beta-hIgGFc transfected HEK293 (2) cell lysate.

Anti-IL1B antibody [3A6] (STJ98171)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of recombinant mouse IL-1 beta protein, using IL1 Beta Rabbit monoclonal antibody (STJ11104780) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-IL1Beta antibody (118-269) [S4780RM] (STJ11104780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of mouse kidney, mouse heart, mouse brain, mouse spleen and rat spleen using IL1F10 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-IL1F10 antibody (101-150 aa) (STJ97678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of MCF7 cells using IL-1RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R1 antibody (31-80 aa) (STJ96628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of PC12 cells using IL-1RII Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R2 antibody (141-190 aa) (STJ96835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ72269 (0. 5µg/ml) staining of LNCaP (A) and (1ug/ml) MOLT-4 (B) cell-lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IL1R2 antibody (Internal) (STJ72269)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-IL1RL1 antibody (251-300 aa) (STJ98813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using IL36R Rabbit polyclonal antibody (STJ112130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IL1RL2 antibody (1-116) (STJ112130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Confocal imaging of mouse tail ear using IL1RA Rabbit monoclonal antibody (STJ11101277, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-IL1RN antibody (100-177) [S7MR] (STJ11101277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of A-431 cells, using IL1RA antibody (STJ24179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1RN antibody (24-177) (STJ24179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using IL1RN Rabbit polyclonal antibody (STJ11104175) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL1RN antibody (27-178) (STJ11104175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using IL-2 Polyclonal Antibody

Anti-IL2 antibody (16-65 aa) (STJ93688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using IL20 antibody (STJ28712). Blue: DAPI for nuclear staining.

Anti-IL20 antibody (25-176) (STJ28712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:200

Anti-IL20 antibody (81-130 aa) (STJ98782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using IL-20R Alpha Polyclonal Antibody

Anti-IL20RA antibody (173-222 aa) (STJ93690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using IL-20R Beta Polyclonal Antibody

Anti-IL20RB antibody (141-190 aa) (STJ93691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL21 antibody (25-162) (STJ29315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of normal 293T cells and 293T transfected with IL22 using IL22 Rabbit mAb (STJ11107003) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Anti-IL22 antibody (34-179 aa) [SRM] (STJ11107003)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of HL-60, using IL22 antibody (STJ27972) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL22 antibody (34-179) (STJ27972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using IL23R Rabbit polyclonal antibody (STJ24181) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL23R antibody (510-629) (STJ24181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 cells using MDA-7 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL24 antibody (131-180 aa) (STJ96776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with 17E/IL25 using IL25 Rabbit pAb (STJ110551) at 1:2000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 90s.

Anti-IL25 antibody (33-177) (STJ110551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200

Anti-IL27 antibody (1-50 aa) (STJ98795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IL2RA antibody (153-167) (STJ24183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IL2RA antibody (223-272 aa) (STJ93693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, CD25 monoclonal antibody (Q22) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL2RA antibody [Q22] (STJ97009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using IL-2R beta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IL2RB antibody (331-380 aa) (STJ93696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela Cell Lysate using antibody. Secondary antibody was diluted at 1:20000

Anti-IL31 antibody (21-70 aa) (STJ98951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using IL-32 Polyclonal Antibody

Anti-IL32 antibody (177-226 aa) (STJ93697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-IL33 antibody (109-270 aa) [SRM] (STJ11107280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of HeLa cells using IL-33 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL33 antibody (121-170 aa) (STJ96852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using IL-34 monoclonal antibody against IL-34-hIgGFc transfected HEK293 cell lysate.

Anti-IL34 antibody [1D12] (STJ98173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200

Anti-IL36A antibody (21-70 aa) (STJ98792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HepG2 cells using IL-1F8 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL36B antibody (111-160 aa) (STJ96877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IL36G antibody (STJ112204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL36G antibody (1-193) (STJ112204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200

Anti-IL37 antibody (101-150 aa) (STJ98774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-IL3R Alpha/CD123 antibody [106C2.02] {Alexa-fluor-488} (STJ16102191)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF

Reactivity :

Human/Pig

£770.50

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Anti-IL3R Alpha/CD123 antibody [106C2.02] {Alexa-fluor-546} (STJ16102192)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF

Reactivity :

Human/Pig

£770.50

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Anti-IL3R Alpha/CD123 antibody [107D2.08] {Alexa-fluor-488} (STJ16102186)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human/Dog

£492.50

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Anti-IL3R Alpha/CD123 antibody [107D2.08] {Alexa-fluor-546} (STJ16102187)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF

Reactivity :

Human/Dog

£492.50

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Anti-IL3R Alpha/CD123 antibody [107D2.08] {Alexa-fluor-647} (STJ16102188)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human/Dog

£492.50

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Western blot analysis of HepG2 cells using IL-3R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL3RA antibody (241-290 aa) (STJ97265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit polyclonal antibody (STJ27616) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-IL4 antibody (25-153) (STJ27616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using IL-4R Alpha Polyclonal Antibody diluted at 1：2000

Anti-IL4R antibody (463-512 aa) (STJ93702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using IL5 Rabbit pAb (STJ110003) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 15s.

Anti-IL5 antibody (20-134) (STJ110003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of recombinant Human IL-5 Protein, using Human IL-5 Rabbit polyclonal antibody (STJ11104218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-IL5 antibody (20-134) (STJ11104218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various cells using IL-5 Polyclonal Antibody

Anti-IL5 antibody (43-92 aa) (STJ93704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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