• Western blot analysis of extracts of various cell lines, using IL36G antibody (STJ112204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of human skin cells using IL36G rabbit polyclonal antibody (STJ112204) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL36G antibody (1-193) (STJ112204)

SKU:
STJ112204

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL36G (1-193) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-193
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of mouse IL36G (NP_705731.2).
Immunogen Sequence: MENNEKKNIVYGSDVEMEHE RAGLFVSSAMFSKHPFSTHI SGRETPDFGEVFDLDQQVWI FRNQALVTVPRSHRVTPVSV TILPCKYPESLEQDKGIAIY LGIQNPDKCLFCKEVNGHPT LLLKEEKILDLYHHPEPMKP FLFYHTRTGGTSTFESVAFP GHYIASSKTGNPIFLTSKKG EYYNINFNLDIKS
Background This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Information sourced from Uniprot.org

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