Porcine Cortisol (Porcine Cortisol) Competitive ELISA Kit (STJE0005370)

SPECIFICATIONS
STJE0005370
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General Information

Short DescriptionThis Cortisol Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in porcine serum and plasma.
ApplicationsELISA
ReactivityPorcine
Sensitivity0.48ng/mL
Detection Limit0.78~50ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time2.5h
DetectionColormetric

Target Information

SpecificityThis kit recognizes Porcine Cortisol in samples. No significant cross-reactivity or interference between Porcine Cortisol and analogues was observed.
Sample TypeSerum and plasma

Additional Info

Specificity This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 81-90 85
EDTA plasma(n=8) 86-97 89
Urine(n=8) 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 50.32 109.34 275.80 51.24 107.22 279.31
Standard deviation 4.14 8.16 20.74 4.59 8.61 21.17
CV (%) 8.23 7.46 7.52 8.95 8.03 7.58

Citations

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