| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
NTH1 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Widely expressed with highest levels in heart and lowest levels in lung and liver. |
| Post Translational Modifications | Ubiquitinated by TRIM26.leading to proteasomal degradation. |
| Function | Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption. |
| Peptide Name | Endonuclease Iii-Like Protein 1Hnth1Bifunctional Dna N-Glycosylase/Dna-(Apurinic Or Apyrimidinic Site LyaseDna Glycosylase/Ap Lyase |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110357Reactome: R-HSA-9630221Reactome: R-HSA-9630222 |
| Cellular Localisation | NucleusMitochondrion |
| Alternative Peptide Names | Endonuclease Iii-Like Protein 1 proteinHnth1 proteinBifunctional Dna N-Glycosylase/Dna-(Apurinic Or Apyrimidinic Site Lyase proteinDna Glycosylase/Ap Lyase proteinNTHL1 proteinNTH1 proteinOCTS3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
