MSX1 Positive Control for STJ501811 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500-1:750
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with MSX1 antibody STJ501811.
Additional Info
Post Translational Modifications
Sumoylated by PIAS1, desumoylated by SENP1. Sumoylation of Lys-15 and Lys-133 not required for interaction with H1-5, transcriptional repression, inhibition of myoblast differentiation, or binding to gene promoters.
Function
Acts as a transcriptional repressor. Capable of transcription autoinactivation. Binds to the consensus sequence 5'-C/GTAAT-3' in downstream activin regulatory elements (DARE) in the gene promoter, thereby repressing the transcription of CGA/alpha-GSU and GNRHR. Represses transcription of myoblast differentiation factors. Binds to core enhancer regions in target gene promoters of myoblast differentiation factors with binding specificity facilitated by interaction with PIAS1. Regulates, in a stage-specific manner, a developmental program of gene expression in the fetal tooth bud that controls odontoblast differentiation and proliferation of dental mesenchymal cells. At the bud stage, required for mesenchymal molar tooth bud development via facilitating reciprocal signaling between dental epithelial and mesenchymal cells. May also regulate expression of Wnt antagonists such as DKK2 and SFPR2 in the developing tooth mesenchyme. Required for BMP4 expression in dental mesenchyme cells. Also, in response to BMP4, required for BMP4 expression in neighboring dental epithelial cells. Required for maximal FGF4-induced expression of SDC1 in dental mesenchyme cells. Also in response to SDC1, required for SDC1 expression in neighboring dental epithelial cells. At the early bell stage, acts to drive proliferation of dental mesenchyme cells, however during the late bell stage acts as an homeostatic regulator of the cell cycle. Regulates proliferation and inhibits premature mesenchymal odontogenesis during the bell stage via inhibition of the Wnt signaling component CTNNB1 and subsequent repression of the odontoblast differentiation factors BMP2, BMP4, LEF1, ALPL and BGLAP/OCN. Additionally, required for correct development and fusion of the palatal shelves and embryonic mandibular formation. Plays a role in embryonic bone formation of the middle ear, skull and nasal bones. Required for correct formation and thickness of the nail plate. May play a role in limb-pattern formation.
Peptide Name
Homeobox Protein Msx-1Homeobox Protein Hox-7Msh Homeobox 1-Like Protein
