MR1 Positive Control for STJ506044 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with MR1 antibody STJ506044.
Additional Info
Tissue Specificity
Ubiquitous. Low expression is detected in peripheral blood B cells, T cells, monocytes and in bronchial epithelial cells (at protein level). Expressed in plasmablasts or plasma B cells in the lamina propria of ileum, appendix and colon (at protein level). Highly expressed on a subset of CD45-positive CD3-positive thymocytes (at protein level).
Post Translational Modifications
N-glycosylated.
Function
Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells. In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers. Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively. May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin. Upon antigen recognition, elicits rapid innate-type MAIT cell activation to eliminate pathogenic microbes by directly killing infected cells. During T cell development, drives thymic selection and post-thymic terminal differentiation of MAIT cells in a process dependent on commensal microflora. Acts as an immune sensor of cancer cell metabolome. May present a tumor-specific or -associated metabolite essential for cancer cell survival to a pan-cancer TCR consisting of TRAV38.2-DV8*TRAJ31 alpha chain paired with a TRBV25.1*TRBJ2.3 beta chain on a non-MAIT CD8-positive T cell clone (MC.7.G5), triggering T cell-mediated killing of a wide range of cancer cell types.
Peptide Name
Major Histocompatibility Complex Class I-Related Gene ProteinMhc Class I-Related Gene ProteinClass I Histocompatibility Antigen-Like Protein
Cellular Localisation
Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneGolgi Apparatus MembraneEarly Endosome MembraneLate Endosome MembraneIn The Absence Of Antigen Remains Within The Endoplasmic Reticulum Where It Acts As A Metabolite SensorAntigen Binding Triggers Trafficking Of The Ternary Complex To The Plasma MembraneAfter PresentationMost Of These Complexes Are Rapidly Internalized And Degraded Via EndocytosisA Small Subset Recycles Via Endosomes Back To The Plasma Membrane And May Thus Acquire And Present New Antigens That Do Not Efficiently Reach The Endoplasmic ReticulumIsoform 1: Cell MembraneSingle-Pass Membrane ProteinIsoform 3: Cell MembraneThe Larger Proportion Remains In The Er In An Immature StateThe Subset That Reach Cell Surface Does It Through A B2m-Independent PathwayIsoform 4: Secreted
Alternative Peptide Names
Major Histocompatibility Complex Class I-Related Gene Protein proteinMhc Class I-Related Gene Protein proteinClass I Histocompatibility Antigen-Like Protein proteinMR1 protein
