| Protein Name | Vip Peptides Cleaved Into - Intestinal Peptide Phi-42 - Intestinal Peptide Phi-27Peptide Histidine Isoleucinamide 27 - Vasoactive Intestinal PeptideVipVasoactive Intestinal Polypeptide |
| Database Links | Reactome: R-MMU-418555Reactome: -MMU-420092 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Vip Peptides Cleaved Into - Intestinal Peptide Phi-42 - Intestinal Peptide Phi-27 ELISA kitPeptide Histidine Isoleucinamide 27 - Vasoactive Intestinal Peptide ELISA kitVip ELISA kitVasoactive Intestinal Polypeptide ELISA kitVip ELISA kit |
| Specificity | This kit recognizes VIP in samples. No significant cross-reactivity or interference between VIP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 92 |
| EDTA plasma(n=8) | 89-102 | 95 |
| Cell culture media(n=8) | 95-105 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 41.60 | 92.10 | 385.20 | 45.70 | 89.50 | 349.50 |
| Standard deviation | 2.50 | 4.90 | 20.00 | 2.50 | 4.90 | 15.00 |
| CV (%) | 6.01 | 5.32 | 5.19 | 5.47 | 5.47 | 4.29 |
