Mouse TREM2 (TREM2) Antibody Pair (STJAP00829)
General Information
| Short Description | This TREM2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R74-6H-3/R29-9E-6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Trem2 |
| Gene ID | 83433 |
| Uniprot ID | TREM2_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in the brain, specifically in microglia (at protein level). Expressed in macrophages (at protein level). Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis. In the CNS not all microglia express TREM2. Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages. |
| Post Translational Modifications | Undergoes ectodomain shedding through proteolytic cleavage by ADAM10 and ADAM17 to produce a transmembrane segment, the TREM2 C-terminal fragment (TREM2-CTF), which is subsequently cleaved by gamma-secretase. |
| Function | Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding. Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia. Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1. Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia. Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin. Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade. Required for microglial phagocytosis of apoptotic neurons. Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain. Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide. It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide.thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB. During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling. Plays a role in microglial MTOR activation and metabolism. Regulates age-related changes in microglial numbers. Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages. In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival. Involved in the positive regulation of osteoclast differentiation. |
| Protein Name | Triggering Receptor Expressed On Myeloid Cells 2Trem-2Triggering Receptor Expressed On Monocytes 2 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-2172127Reactome: -MMU-2424491Reactome: -MMU-416700 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative Antibody Pair Names | Triggering Receptor Expressed On Myeloid Cells 2 antibody pairTrem-2 antibody pairTriggering Receptor Expressed On Monocytes 2 antibody pairTrem2 antibody pairTrem2a antibody pairTrem2b antibody pairTrem2c antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse TREM2 (TREM2) Antibody Pair (STJAP00829)
This antibody pair detects mouse TREM2 (Triggering Receptor Expressed on Myeloid Cells 2), corresponding to UniProt entry Q99NH8. Reactivity has been confirmed for mouse only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. Clone IDs are R74-6H-3 and R29-9E-6, and both have been affinity purified.
This antibody pair is validated for use in ELISA. No other assay formats are specified for this product.
Both antibodies were raised against a recombinant protein immunogen corresponding to mouse TREM2. No further detail on the specific immunogen region is provided in the current product specification.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Working dilutions for both the capture and detection antibodies should be determined by the end user, as no fixed dilution range is specified for this product. Titration experiments are recommended to establish optimal concentrations for each specific experimental setup.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.