| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
| Database Links | Reactome: R-MMU-390522Reactome: -MMU-5578775 |
| Alternative ELISA Names | Troponin I - Cardiac Muscle ELISA kitCardiac Troponin I ELISA kitTnni3 ELISA kit |
| Specificity | This kit recognizes Mouse TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Mouse TNNI3/cTn-I and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 94 |
| EDTA plasma (n=8) | 86-101 | 93 |
| Cell culture media (n=8) | 92-107 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 25.50 | 62.70 | 175.10 | 24.70 | 60.50 | 162.00 |
| Standard deviation | 1.70 | 3.00 | 8.10 | 1.60 | 3.10 | 8.30 |
| CV (%) | 6.67 | 4.78 | 4.63 | 6.48 | 5.12 | 5.12 |
