This Tjp1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Mouse
Sensitivity
46.88pg/mL
Detection Limit
78.13~5000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Mouse TJP1 in samples. No significant cross-reactivity or interference between Mouse TJP1 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.
Function
Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With Tjp2 and TJjp3, participates in the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions.
Protein Name
Tight Junction Protein 1Tight Junction Protein Zo-1Zona Occludens Protein 1Zonula Occludens Protein 1
Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionTight JunctionGap JunctionCytoplasmMyofibrilSarcomereI BandMoves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell ContactForms A Condensed Tight Junction-Linked Belt Of Protein During Junction Formation Which Becomes Anchored To The Apical Cell Membrane Via Interaction With PatjDetected At The Leading Edge Of Migrating And Wounded CellsColocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Alternative ELISA Names
Tight Junction Protein 1 ELISA kitTight Junction Protein Zo-1 ELISA kitZona Occludens Protein 1 ELISA kitZonula Occludens Protein 1 ELISA kitTjp1 ELISA kitZo1 ELISA kit
Specificity
This kit recognizes Mouse TJP1 in samples. No significant cross-reactivity or interference between Mouse TJP1 and analogues was observed.
