This Surv Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates, cell culture supernates or other biological fluids..
Applications
ELISA
Reactivity
Mouse
Sensitivity
5.6pg/mL
Detection Limit
15.6-1000pg/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Additional Info
Tissue Specificity
Post Translational Modifications
Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting. Deubiquitinated by USP35 or USP38.leading to stabilization. Acetylation at Lys-129 results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation. In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities. Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity.
Function
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex.directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function.
Protein Name
Baculoviral Iap Repeat-Containing Protein 5Apoptosis Inhibitor 4Apoptosis Inhibitor SurvivinTiap
Database Links
Cellular Localisation
CytoplasmNucleusChromosomeCentromereCytoskeletonSpindleKinetochoreMidbodyLocalizes At The Centromeres From Prophase To MetaphaseAt The Spindle Midzone During Anaphase And A The Midbody During Telophase And CytokinesisAccumulates In The Nucleus Upon Treatment With Leptomycin B (Lmb)A Xpo1/Crm1 Nuclear Export InhibitorLocalizes On Chromosome Arms And Inner Centromeres From Prophase Through MetaphaseLocalizes To Kinetochores In MetaphaseDistributes To The Midzone Microtubules In Anaphase And At TelophaseLocalizes Exclusively To The MidbodyColocalizes With Aurkb At Mitotic ChromosomesAcetylation At Lys-129 Directs Its Localization To The Nucleus By Enhancing Homodimerization And Thereby Inhibiting Xpo1/Crm1-Mediated Nuclear Export
