Mouse Soluble CD4 protein (Recombinant) (STJP000364)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Mouse CD4 extracellular domain sp|P06332|27-394 was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Mouse Soluble CD4-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered solution in PBS, pH7.4. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by immobilized recombinant mouse CD4 (1ug/ml) ability to support adhesion of HeLa cells (5x104 cells/well ) ,-80% will adhere after 20min at4C. NA |
Target Information
| Gene Symbol | Cd4 |
| Gene ID | 12504 |
| Uniprot ID | CD4_MOUSE |
| Immunogen | Optimized DNA sequence encoding Mouse CD4 extracellular domain sp|P06332|27-394 was expressed in HEK293 cells. NA |
| Immunogen Region | 27-394aa, ECD |
| Immunogen Sequence | MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFT |
Additional Info
| Tissue Specificity | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
| Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation plays an important role for CD4 internalization. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
| Protein Name | T-Cell Surface Glycoprotein Cd4T-Cell Differentiation Antigen L3t4T-Cell Surface Antigen T4/Leu-3Cd Antigen Cd4 |
| Database Links | Reactome: R-MMU-1462054Reactome: -MMU-202424Reactome: -MMU-202427Reactome: -MMU-202430Reactome: -MMU-202433Reactome: -MMU-389948Reactome: -MMU-449836Reactome: -MMU-8856825Reactome: -MMU-8856828 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Cell Surface Glycoprotein Cd4 proteinT-Cell Differentiation Antigen L3t4 proteinT-Cell Surface Antigen T4/Leu-3 proteinCd Antigen Cd4 proteinCd4 protein |
Information sourced from Uniprot.org