Mouse Slc6a3 (Sodium-dependent dopamine transporter) Sandwich ELISA Kit (STJE0004514)

SPECIFICATIONS
STJE0004514
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General Information

Short DescriptionThis Slc6a3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityMouse
Sensitivity4.69pg/mL
Detection Limit7.81~500pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolSlc6a3
Gene ID13162
Uniprot IDSC6A3_MOUSE
SpecificityThis kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Mediates sodium- and chloride-dependent transport of dopamine. Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling.
Protein Name Sodium-Dependent Dopamine Transporter
Da Transporter
Dat
Solute Carrier Family 6 Member 3
Database Links Reactome: R-MMU-379401
Reactome: -MMU-442660
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Neuron Projection
Axon
Localizes To Neurite Tips In Neuronal Cells
Colocalizes With Septin4 At Axon Terminals
Especially At The Varicosities
Alternative ELISA Names Sodium-Dependent Dopamine Transporter ELISA kit
Da Transporter ELISA kit
Dat ELISA kit
Solute Carrier Family 6 Member 3 ELISA kit
Slc6a3 ELISA kit
Dat ELISA kit
Dat1 ELISA kit
Specificity This kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 98
EDTA plasma (n=8) 84-97 91
Cell culture media (n=8) 85-99 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 22.94 71.32 212.65 23.07 72.20 200.28
Standard deviation 1.22 3.16 9.25 1.53 3.42 10.59
CV (%) 5.32 4.43 4.35 6.63 4.74 5.29

Citations

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