Mouse SCF (Kit Ligand) Antibody Pair (STJAP00311)
General Information
| Short Description | This SCF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Kitlg |
| Gene ID | 17311 |
| Uniprot ID | SCF_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse SCF protein.Detection Immunogen: Recombinant Mouse SCF protein. |
Additional Info
| Post Translational Modifications | A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain. |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Protein Name | Kit LigandHematopoietic Growth Factor KlMast Cell Growth FactorMgfSteel FactorStem Cell FactorScfC-Kit Ligand Cleaved Into - Soluble Kit LigandSkitlg |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-1433557Reactome: -MMU-1433559Reactome: -MMU-5673001Reactome: -MMU-6811558Reactome: -MMU-9856649 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: CytoplasmCytoplasmCytoskeletonCell MembraneCell ProjectionLamellipodiumFilopodiumSoluble Kit Ligand: Secreted |
| Alternative Antibody Pair Names | Kit Ligand antibody pairHematopoietic Growth Factor Kl antibody pairMast Cell Growth Factor antibody pairMgf antibody pairSteel Factor antibody pairStem Cell Factor antibody pairScf antibody pairC-Kit Ligand Cleaved Into - Soluble Kit Ligand antibody pairSkitlg antibody pairKitlg antibody pairKitl antibody pairMgf antibody pairSl antibody pairSlf antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse SCF (Kit Ligand) Antibody Pair (STJAP00311)
This antibody pair detects mouse SCF (Stem Cell Factor, also known as Kit Ligand), corresponding to UniProt entry P20826. Reactivity is confirmed for mouse only.
Both antibodies are polyclonal, raised in goat, and purified by antigen affinity. The capture antibody is unconjugated, while the detection antibody is biotinylated. Each component is supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant mouse SCF protein. No additional immunogen region details are specified.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.