Mouse S100a8 (Protein S100-A8) Sandwich ELISA Kit (STJE0005364)

Name: Mouse S100a8 (Protein S100-A8) Sandwich ELISA Kit (STJE0005364)
SKU: STJE0005364
Availability: InStock

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SPECIFICATIONS

STJE0005364

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Short Description	This S100a8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	S100a8
Gene ID	20201
Uniprot ID	S10A8_MOUSE
Specificity	This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Protein Name	Protein S100-A8 Calgranulin-A Chemotactic Cytokine Cp-10 Leukocyte L1 Complex Light Chain Migration Inhibitory Factor-Related Protein 8 Mrp-8 P8 Pro-Inflammatory S100 Cytokine S100 Calcium-Binding Protein A8
Database Links	Reactome: R-MMU-5668599 Reactome: -MMU-5686938 Reactome: -MMU-6798695 Reactome: -MMU-6799990
Cellular Localisation	Secreted Cytoplasm Cytoskeleton Cell Membrane Peripheral Membrane Protein Predominantly Localized In The Cytoplasm Upon Elevation Of The Intracellular Calcium Level Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane Upon Neutrophil Activation Or Endothelial Adhesion Of Monocytes Is Secreted Via A Microtubule-Mediated Alternative Pathway
Alternative ELISA Names	Protein S100-A8 ELISA kit Calgranulin-A ELISA kit Chemotactic Cytokine Cp-10 ELISA kit Leukocyte L1 Complex Light Chain ELISA kit Migration Inhibitory Factor-Related Protein 8 ELISA kit Mrp-8 ELISA kit P8 ELISA kit Pro-Inflammatory S100 Cytokine ELISA kit S100 Calcium-Binding Protein A8 ELISA kit S100a8 ELISA kit Caga ELISA kit Mrp8 ELISA kit
Specificity	This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	188.64	414.53	1934.43	195.77	446.65	1946.95
Standard deviation	11.11	20.60	92.08	11.39	20.64	78.07
CV (%)	5.89	4.97	4.76	5.82	4.62	4.01