Mouse RAN Kappa L (Tumor Necrosis Factor Ligand Superfamily Member 11) Antibody Pair (STJAP00541)
General Information
| Short Description | This RAN Kappa L antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Tnfsf11 |
| Gene ID | 21943 |
| Uniprot ID | TNF11_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse RAN Kappa L protien.Detection Immunogen: Recombinant Mouse RAN Kappa L protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing. The cleavage may be catalyzed by ADAM17. A further shorter soluble form was observed. |
| Function | Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation. |
| Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 11Osteoclast Differentiation FactorOdfOsteoprotegerin LigandOpglReceptor Activator Of Nuclear Factor Kappa-B LigandRanklTnf-Related Activation-Induced CytokineTranceCd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form |
| Database Links | Reactome: R-MMU-5668541Reactome: -MMU-5669034Reactome: -MMU-5676594Reactome: -MMU-9856649 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type Ii Membrane ProteinIsoform 2: Cell MembraneIsoform 3: CytoplasmTumor Necrosis Factor Ligand Superfamily Member 11Soluble Form: Secreted |
| Alternative Antibody Pair Names | Tumor Necrosis Factor Ligand Superfamily Member 11 antibody pairOsteoclast Differentiation Factor antibody pairOdf antibody pairOsteoprotegerin Ligand antibody pairOpgl antibody pairReceptor Activator Of Nuclear Factor Kappa-B Ligand antibody pairRankl antibody pairTnf-Related Activation-Induced Cytokine antibody pairTrance antibody pairCd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form antibody pairTnfsf11 antibody pairOpgl antibody pairRankl antibody pairTrance antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse RAN Kappa L (Tumor Necrosis Factor Ligand Superfamily Member 11) Antibody Pair (STJAP00541)
This antibody pair detects Mouse RAN Kappa L, also known as Tumor Necrosis Factor Ligand Superfamily Member 11 (UniProt O35235), and has confirmed reactivity to mouse. It is not validated for detection of this target in other species.
The capture antibody is supplied unconjugated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both antibodies are supplied at 0.5 mg/mL and are raised in goat.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were purified by antigen affinity chromatography using recombinant Mouse RAN Kappa L protein as the immunogen for each.
This antibody pair is suitable for use with serum, plasma, and other biological fluids of mouse origin. This product is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.