This Ppia Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Mouse
Sensitivity
0.09ng/mL
Detection Limit
0.16~10ng/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Mouse CYPA in samples. No significant cross-reactivity or interference between Mouse CYPA and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Acetylation at Lys-125 markedly inhibits catalysis of cis to trans isomerization. PPIA acetylation also antagonizes the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition. Acetylation at Lys-125 favors the interaction with TARDBP.
Function
Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2. Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1. Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates. Plays an important role in platelet activation and aggregation. Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton. Binds heparan sulfate glycosaminoglycans.
Protein Name
Peptidyl-Prolyl Cis-Trans Isomerase APpiase ACyclophilin ACyclosporin A-Binding ProteinRotamase ASp18 Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed
CytoplasmSecretedNucleusSecretion Occurs In Response To Oxidative Stress In Vascular Smooth Muscle Through A Vesicular Secretory Pathway That Involves Actin Remodeling And Myosin Ii ActivationAnd Mediates Erk1/2 Activation
Alternative ELISA Names
Peptidyl-Prolyl Cis-Trans Isomerase A ELISA kitPpiase A ELISA kitCyclophilin A ELISA kitCyclosporin A-Binding Protein ELISA kitRotamase A ELISA kitSp18 Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed ELISA kitPpia ELISA kit
Specificity
This kit recognizes Mouse CYPA in samples. No significant cross-reactivity or interference between Mouse CYPA and analogues was observed.
