Mouse POSTN (Periostin) Sandwich ELISA Kit (STJE0019977)

SPECIFICATIONS
STJE0019977
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General Information

Short DescriptionThis POSTN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityMouse
Sensitivity0.45 ng/mL
Detection Limit0.78-50 ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolPostn
Gene ID50706
Uniprot IDPOSTN_MOUSE
SpecificityThis kit recognizes Mouse POSTN in samples. No significant cross-reactivity or interference between Mouse POSTN and analogues was observed.
Sample Typeserum, plasma and other biological fluids

Additional Info

Tissue Specificity Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart.
Post Translational Modifications Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report in human, does not contain vitamin K-dependent gamma-carboxyglutamate residues.
Function Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
Protein Name Periostin
Pn
Osteoblast-Specific Factor 2
Osf-2
Database Links
Cellular Localisation Golgi Apparatus
Secreted
Extracellular Space
Extracellular Matrix
Colocalizes With Bmp1 In The Golgi
Alternative ELISA Names Periostin ELISA kit
Pn ELISA kit
Osteoblast-Specific Factor 2 ELISA kit
Osf-2 ELISA kit
Postn ELISA kit
Osf2 ELISA kit
Specificity This kit recognizes Mouse POSTN in samples. No significant cross-reactivity or interference between Mouse POSTN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-109 101
EDTA plasma (n=8) 92-106 99
Cell culture media (n=8) 85-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.57 6.03 20.97 2.35 6.33 19.21
Standard deviation 0.17 0.31 0.95 0.14 0.31 0.63
CV (%) 6.72 5.18 4.51 5.78 4.88 3.28

Citations

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